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Unique molecular events during reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) at naïve state

Derivation of human naïve cells in the ground state of pluripotency provides promising avenues for developmental biology studies and therapeutic manipulations. However, the molecular mechanisms involved in the establishment and maintenance of human naïve pluripotency remain poorly understood. Using...

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Autores principales: Wang, Yixuan, Zhao, Chengchen, Hou, Zhenzhen, Yang, Yuanyuan, Bi, Yan, Wang, Hong, Zhang, Yong, Gao, Shaorong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5807049/
https://www.ncbi.nlm.nih.gov/pubmed/29381138
http://dx.doi.org/10.7554/eLife.29518
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author Wang, Yixuan
Zhao, Chengchen
Hou, Zhenzhen
Yang, Yuanyuan
Bi, Yan
Wang, Hong
Zhang, Yong
Gao, Shaorong
author_facet Wang, Yixuan
Zhao, Chengchen
Hou, Zhenzhen
Yang, Yuanyuan
Bi, Yan
Wang, Hong
Zhang, Yong
Gao, Shaorong
author_sort Wang, Yixuan
collection PubMed
description Derivation of human naïve cells in the ground state of pluripotency provides promising avenues for developmental biology studies and therapeutic manipulations. However, the molecular mechanisms involved in the establishment and maintenance of human naïve pluripotency remain poorly understood. Using the human inducible reprogramming system together with the 5iLAF naïve induction strategy, integrative analysis of transcriptional and epigenetic dynamics across the transition from human fibroblasts to naïve iPSCs revealed ordered waves of gene network activation sharing signatures with those found during embryonic development from late embryogenesis to pre-implantation stages. More importantly, Transcriptional analysis showed a significant transient reactivation of transcripts with 8-cell-stage-like characteristics in the late stage of reprogramming, suggesting transient activation of gene network with human zygotic genome activation (ZGA)-like signatures during the establishment of naïve pluripotency. Together, Dissecting the naïve reprogramming dynamics by integrative analysis improves the understanding of the molecular features involved in the generation of naïve pluripotency directly from somatic cells.
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spelling pubmed-58070492018-02-12 Unique molecular events during reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) at naïve state Wang, Yixuan Zhao, Chengchen Hou, Zhenzhen Yang, Yuanyuan Bi, Yan Wang, Hong Zhang, Yong Gao, Shaorong eLife Stem Cells and Regenerative Medicine Derivation of human naïve cells in the ground state of pluripotency provides promising avenues for developmental biology studies and therapeutic manipulations. However, the molecular mechanisms involved in the establishment and maintenance of human naïve pluripotency remain poorly understood. Using the human inducible reprogramming system together with the 5iLAF naïve induction strategy, integrative analysis of transcriptional and epigenetic dynamics across the transition from human fibroblasts to naïve iPSCs revealed ordered waves of gene network activation sharing signatures with those found during embryonic development from late embryogenesis to pre-implantation stages. More importantly, Transcriptional analysis showed a significant transient reactivation of transcripts with 8-cell-stage-like characteristics in the late stage of reprogramming, suggesting transient activation of gene network with human zygotic genome activation (ZGA)-like signatures during the establishment of naïve pluripotency. Together, Dissecting the naïve reprogramming dynamics by integrative analysis improves the understanding of the molecular features involved in the generation of naïve pluripotency directly from somatic cells. eLife Sciences Publications, Ltd 2018-01-30 /pmc/articles/PMC5807049/ /pubmed/29381138 http://dx.doi.org/10.7554/eLife.29518 Text en © 2018, Wang et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Stem Cells and Regenerative Medicine
Wang, Yixuan
Zhao, Chengchen
Hou, Zhenzhen
Yang, Yuanyuan
Bi, Yan
Wang, Hong
Zhang, Yong
Gao, Shaorong
Unique molecular events during reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) at naïve state
title Unique molecular events during reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) at naïve state
title_full Unique molecular events during reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) at naïve state
title_fullStr Unique molecular events during reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) at naïve state
title_full_unstemmed Unique molecular events during reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) at naïve state
title_short Unique molecular events during reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) at naïve state
title_sort unique molecular events during reprogramming of human somatic cells to induced pluripotent stem cells (ipscs) at naïve state
topic Stem Cells and Regenerative Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5807049/
https://www.ncbi.nlm.nih.gov/pubmed/29381138
http://dx.doi.org/10.7554/eLife.29518
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