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Coupling the recombineering to Cre-lox system enables simplified large-scale genome deletion in Lactobacillus casei
BACKGROUND: Lactobacillus casei is widely used in the dairy and pharmaceutical industries and a promising candidate for use as cell factories. Recently, genome sequencing and functional genomics provide the possibility for reducing L. casei genome. However, it was still limited by the inefficient an...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5808424/ https://www.ncbi.nlm.nih.gov/pubmed/29433512 http://dx.doi.org/10.1186/s12934-018-0872-4 |