High throughput protease profiling comprehensively defines active site specificity for thrombin and ADAMTS13

We have combined random 6 amino acid substrate phage display with high throughput sequencing to comprehensively define the active site specificity of the serine protease thrombin and the metalloprotease ADAMTS13. The substrate motif for thrombin was determined by >6,700 cleaved peptides, and was...

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Detalles Bibliográficos
Autores principales: Kretz, Colin A., Tomberg, Kärt, Van Esbroeck, Alexander, Yee, Andrew, Ginsburg, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809430/
https://www.ncbi.nlm.nih.gov/pubmed/29434246
http://dx.doi.org/10.1038/s41598-018-21021-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809430/
https://www.ncbi.nlm.nih.gov/pubmed/29434246
http://dx.doi.org/10.1038/s41598-018-21021-9

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