Next-Generation Sequencing of Antibody Display Repertoires

In vitro selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high affinity binders can be selected from diverse repertoires. Here, we review strategies for the next-generation sequencing (NGS) of phage-...

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Detalles Bibliográficos
Autores principales: Rouet, Romain, Jackson, Katherine J. L., Langley, David B., Christ, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810246/
https://www.ncbi.nlm.nih.gov/pubmed/29472918
http://dx.doi.org/10.3389/fimmu.2018.00118
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author Rouet, Romain
Jackson, Katherine J. L.
Langley, David B.
Christ, Daniel
author_facet Rouet, Romain
Jackson, Katherine J. L.
Langley, David B.
Christ, Daniel
author_sort Rouet, Romain
collection PubMed
description In vitro selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high affinity binders can be selected from diverse repertoires. Here, we review strategies for the next-generation sequencing (NGS) of phage- and other antibody-display libraries, as well as NGS platforms and analysis tools. Moreover, we discuss recent examples relating to the use of NGS to assess library diversity, clonal enrichment, and affinity maturation.
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spelling pubmed-58102462018-02-22 Next-Generation Sequencing of Antibody Display Repertoires Rouet, Romain Jackson, Katherine J. L. Langley, David B. Christ, Daniel Front Immunol Immunology In vitro selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high affinity binders can be selected from diverse repertoires. Here, we review strategies for the next-generation sequencing (NGS) of phage- and other antibody-display libraries, as well as NGS platforms and analysis tools. Moreover, we discuss recent examples relating to the use of NGS to assess library diversity, clonal enrichment, and affinity maturation. Frontiers Media S.A. 2018-02-02 /pmc/articles/PMC5810246/ /pubmed/29472918 http://dx.doi.org/10.3389/fimmu.2018.00118 Text en Copyright © 2018 Rouet, Jackson, Langley and Christ. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Rouet, Romain
Jackson, Katherine J. L.
Langley, David B.
Christ, Daniel
Next-Generation Sequencing of Antibody Display Repertoires
title Next-Generation Sequencing of Antibody Display Repertoires
title_full Next-Generation Sequencing of Antibody Display Repertoires
title_fullStr Next-Generation Sequencing of Antibody Display Repertoires
title_full_unstemmed Next-Generation Sequencing of Antibody Display Repertoires
title_short Next-Generation Sequencing of Antibody Display Repertoires
title_sort next-generation sequencing of antibody display repertoires
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810246/
https://www.ncbi.nlm.nih.gov/pubmed/29472918
http://dx.doi.org/10.3389/fimmu.2018.00118
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