Enzyme-mediated single-nucleotide variation detection at room temperature with high discrimination factor

We demonstrate a new powerful tool to detect single-nucleotide variation in DNA at room temperature with high selectivity, based on predetermined specific interactions between Lambda exonuclease and a chemically modified DNA substrate structure which comprises two purposefully introduced mismatches...

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Detalles Bibliográficos
Autores principales: Wu, Tongbo, Xiao, Xianjin, Zhang, Zhe, Zhao, Meiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811150/
https://www.ncbi.nlm.nih.gov/pubmed/29560206
http://dx.doi.org/10.1039/c4sc03375b

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811150/
https://www.ncbi.nlm.nih.gov/pubmed/29560206
http://dx.doi.org/10.1039/c4sc03375b

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