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Enzyme-mediated single-nucleotide variation detection at room temperature with high discrimination factor
We demonstrate a new powerful tool to detect single-nucleotide variation in DNA at room temperature with high selectivity, based on predetermined specific interactions between Lambda exonuclease and a chemically modified DNA substrate structure which comprises two purposefully introduced mismatches...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Royal Society of Chemistry
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811150/ https://www.ncbi.nlm.nih.gov/pubmed/29560206 http://dx.doi.org/10.1039/c4sc03375b |