Automation aided optimization of cloning, expression and purification of enzymes of the bacterial sialic acid catabolic and sialylation pathways enzymes for structural studies

The process of obtaining a well‐expressing, soluble and correctly folded constructs can be made easier and quicker by automating the optimization of cloning, expression and purification. While there are many semiautomated pipelines available for cloning, expression and purification, there is hardly...

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Detalles Bibliográficos
Autores principales: Bairy, Sneha, Gopalan, Lakshmi Narayanan, Setty, Thanuja Gangi, Srinivasachari, Sathya, Manjunath, Lavanyaa, Kumar, Jay Prakash, Guntupalli, Sai R, Bose, Sucharita, Nayak, Vinod, Ghosh, Swagatha, Sathyanarayanan, Nitish, Caing‐Carlsson, Rhawnie, Wahlgren, Weixiao Yuan, Friemann, Rosmarie, Ramaswamy, S., Neerathilingam, Muniasamy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812244/
https://www.ncbi.nlm.nih.gov/pubmed/29345069
http://dx.doi.org/10.1111/1751-7915.13041

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812244/
https://www.ncbi.nlm.nih.gov/pubmed/29345069
http://dx.doi.org/10.1111/1751-7915.13041

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