Rational cell culture optimization enhances experimental reproducibility in cancer cells

Optimization of experimental conditions is critical in ensuring robust experimental reproducibility. Through detailed metabolomic analysis we found that cell culture conditions significantly impacted on glutaminase (GLS1) sensitivity resulting in variable sensitivity and irreproducibility in data. B...

Descripción completa

Detalles Bibliográficos
Autores principales: Wright Muelas, Marina, Ortega, Fernando, Breitling, Rainer, Bendtsen, Claus, Westerhoff, Hans V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813001/
https://www.ncbi.nlm.nih.gov/pubmed/29445172
http://dx.doi.org/10.1038/s41598-018-21050-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813001/
https://www.ncbi.nlm.nih.gov/pubmed/29445172
http://dx.doi.org/10.1038/s41598-018-21050-4

Ejemplares similares