Directional freezing for the cryopreservation of adherent mammalian cells on a substrate

Successfully cryopreserving cells adhered to a substrate would facilitate the growth of a vital confluent cell culture after thawing while dramatically shortening the post-thaw culturing time. Herein we propose a controlled slow cooling method combining initial directional freezing followed by gradu...

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Detalles Bibliográficos
Autores principales: Bahari, Liat, Bein, Amir, Yashunsky, Victor, Braslavsky, Ido
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813933/
https://www.ncbi.nlm.nih.gov/pubmed/29447224
http://dx.doi.org/10.1371/journal.pone.0192265

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813933/
https://www.ncbi.nlm.nih.gov/pubmed/29447224
http://dx.doi.org/10.1371/journal.pone.0192265

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