Cargando…
Directional freezing for the cryopreservation of adherent mammalian cells on a substrate
Successfully cryopreserving cells adhered to a substrate would facilitate the growth of a vital confluent cell culture after thawing while dramatically shortening the post-thaw culturing time. Herein we propose a controlled slow cooling method combining initial directional freezing followed by gradu...
| Autores principales: | , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2018
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813933/ https://www.ncbi.nlm.nih.gov/pubmed/29447224 http://dx.doi.org/10.1371/journal.pone.0192265 |