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Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring
We investigated maternal obesity in inbred SM/J mice by assigning females to a high-fat diet or a low-fat diet at weaning, mating them to low-fat-fed males, cross-fostering the offspring to low-fat-fed SM/J nurses at birth, and weaning the offspring onto a high-fat or low-fat diet. A maternal high-f...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813940/ https://www.ncbi.nlm.nih.gov/pubmed/29447215 http://dx.doi.org/10.1371/journal.pone.0192606 |
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author | Keleher, Madeline Rose Zaidi, Rabab Shah, Shyam Oakley, M. Elsa Pavlatos, Cassondra El Idrissi, Samir Xing, Xiaoyun Li, Daofeng Wang, Ting Cheverud, James M. |
author_facet | Keleher, Madeline Rose Zaidi, Rabab Shah, Shyam Oakley, M. Elsa Pavlatos, Cassondra El Idrissi, Samir Xing, Xiaoyun Li, Daofeng Wang, Ting Cheverud, James M. |
author_sort | Keleher, Madeline Rose |
collection | PubMed |
description | We investigated maternal obesity in inbred SM/J mice by assigning females to a high-fat diet or a low-fat diet at weaning, mating them to low-fat-fed males, cross-fostering the offspring to low-fat-fed SM/J nurses at birth, and weaning the offspring onto a high-fat or low-fat diet. A maternal high-fat diet exacerbated obesity in the high-fat-fed daughters, causing them to weigh more, have more fat, and have higher serum levels of leptin as adults, accompanied by dozens of gene expression changes and thousands of DNA methylation changes in their livers and hearts. Maternal diet particularly affected genes involved in RNA processing, immune response, and mitochondria. Between one-quarter and one-third of differentially expressed genes contained a differentially methylated region associated with maternal diet. An offspring high-fat diet reduced overall variation in DNA methylation, increased body weight and organ weights, increased long bone lengths and weights, decreased insulin sensitivity, and changed the expression of 3,908 genes in the liver. Although the offspring were more affected by their own diet, their maternal diet had epigenetic effects lasting through adulthood, and in the daughters these effects were accompanied by phenotypic changes relevant to obesity and diabetes. |
format | Online Article Text |
id | pubmed-5813940 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58139402018-03-02 Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring Keleher, Madeline Rose Zaidi, Rabab Shah, Shyam Oakley, M. Elsa Pavlatos, Cassondra El Idrissi, Samir Xing, Xiaoyun Li, Daofeng Wang, Ting Cheverud, James M. PLoS One Research Article We investigated maternal obesity in inbred SM/J mice by assigning females to a high-fat diet or a low-fat diet at weaning, mating them to low-fat-fed males, cross-fostering the offspring to low-fat-fed SM/J nurses at birth, and weaning the offspring onto a high-fat or low-fat diet. A maternal high-fat diet exacerbated obesity in the high-fat-fed daughters, causing them to weigh more, have more fat, and have higher serum levels of leptin as adults, accompanied by dozens of gene expression changes and thousands of DNA methylation changes in their livers and hearts. Maternal diet particularly affected genes involved in RNA processing, immune response, and mitochondria. Between one-quarter and one-third of differentially expressed genes contained a differentially methylated region associated with maternal diet. An offspring high-fat diet reduced overall variation in DNA methylation, increased body weight and organ weights, increased long bone lengths and weights, decreased insulin sensitivity, and changed the expression of 3,908 genes in the liver. Although the offspring were more affected by their own diet, their maternal diet had epigenetic effects lasting through adulthood, and in the daughters these effects were accompanied by phenotypic changes relevant to obesity and diabetes. Public Library of Science 2018-02-15 /pmc/articles/PMC5813940/ /pubmed/29447215 http://dx.doi.org/10.1371/journal.pone.0192606 Text en © 2018 Keleher et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Keleher, Madeline Rose Zaidi, Rabab Shah, Shyam Oakley, M. Elsa Pavlatos, Cassondra El Idrissi, Samir Xing, Xiaoyun Li, Daofeng Wang, Ting Cheverud, James M. Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring |
title | Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring |
title_full | Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring |
title_fullStr | Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring |
title_full_unstemmed | Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring |
title_short | Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring |
title_sort | maternal high-fat diet associated with altered gene expression, dna methylation, and obesity risk in mouse offspring |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813940/ https://www.ncbi.nlm.nih.gov/pubmed/29447215 http://dx.doi.org/10.1371/journal.pone.0192606 |
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