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Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring

We investigated maternal obesity in inbred SM/J mice by assigning females to a high-fat diet or a low-fat diet at weaning, mating them to low-fat-fed males, cross-fostering the offspring to low-fat-fed SM/J nurses at birth, and weaning the offspring onto a high-fat or low-fat diet. A maternal high-f...

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Autores principales: Keleher, Madeline Rose, Zaidi, Rabab, Shah, Shyam, Oakley, M. Elsa, Pavlatos, Cassondra, El Idrissi, Samir, Xing, Xiaoyun, Li, Daofeng, Wang, Ting, Cheverud, James M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813940/
https://www.ncbi.nlm.nih.gov/pubmed/29447215
http://dx.doi.org/10.1371/journal.pone.0192606
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author Keleher, Madeline Rose
Zaidi, Rabab
Shah, Shyam
Oakley, M. Elsa
Pavlatos, Cassondra
El Idrissi, Samir
Xing, Xiaoyun
Li, Daofeng
Wang, Ting
Cheverud, James M.
author_facet Keleher, Madeline Rose
Zaidi, Rabab
Shah, Shyam
Oakley, M. Elsa
Pavlatos, Cassondra
El Idrissi, Samir
Xing, Xiaoyun
Li, Daofeng
Wang, Ting
Cheverud, James M.
author_sort Keleher, Madeline Rose
collection PubMed
description We investigated maternal obesity in inbred SM/J mice by assigning females to a high-fat diet or a low-fat diet at weaning, mating them to low-fat-fed males, cross-fostering the offspring to low-fat-fed SM/J nurses at birth, and weaning the offspring onto a high-fat or low-fat diet. A maternal high-fat diet exacerbated obesity in the high-fat-fed daughters, causing them to weigh more, have more fat, and have higher serum levels of leptin as adults, accompanied by dozens of gene expression changes and thousands of DNA methylation changes in their livers and hearts. Maternal diet particularly affected genes involved in RNA processing, immune response, and mitochondria. Between one-quarter and one-third of differentially expressed genes contained a differentially methylated region associated with maternal diet. An offspring high-fat diet reduced overall variation in DNA methylation, increased body weight and organ weights, increased long bone lengths and weights, decreased insulin sensitivity, and changed the expression of 3,908 genes in the liver. Although the offspring were more affected by their own diet, their maternal diet had epigenetic effects lasting through adulthood, and in the daughters these effects were accompanied by phenotypic changes relevant to obesity and diabetes.
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spelling pubmed-58139402018-03-02 Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring Keleher, Madeline Rose Zaidi, Rabab Shah, Shyam Oakley, M. Elsa Pavlatos, Cassondra El Idrissi, Samir Xing, Xiaoyun Li, Daofeng Wang, Ting Cheverud, James M. PLoS One Research Article We investigated maternal obesity in inbred SM/J mice by assigning females to a high-fat diet or a low-fat diet at weaning, mating them to low-fat-fed males, cross-fostering the offspring to low-fat-fed SM/J nurses at birth, and weaning the offspring onto a high-fat or low-fat diet. A maternal high-fat diet exacerbated obesity in the high-fat-fed daughters, causing them to weigh more, have more fat, and have higher serum levels of leptin as adults, accompanied by dozens of gene expression changes and thousands of DNA methylation changes in their livers and hearts. Maternal diet particularly affected genes involved in RNA processing, immune response, and mitochondria. Between one-quarter and one-third of differentially expressed genes contained a differentially methylated region associated with maternal diet. An offspring high-fat diet reduced overall variation in DNA methylation, increased body weight and organ weights, increased long bone lengths and weights, decreased insulin sensitivity, and changed the expression of 3,908 genes in the liver. Although the offspring were more affected by their own diet, their maternal diet had epigenetic effects lasting through adulthood, and in the daughters these effects were accompanied by phenotypic changes relevant to obesity and diabetes. Public Library of Science 2018-02-15 /pmc/articles/PMC5813940/ /pubmed/29447215 http://dx.doi.org/10.1371/journal.pone.0192606 Text en © 2018 Keleher et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Keleher, Madeline Rose
Zaidi, Rabab
Shah, Shyam
Oakley, M. Elsa
Pavlatos, Cassondra
El Idrissi, Samir
Xing, Xiaoyun
Li, Daofeng
Wang, Ting
Cheverud, James M.
Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring
title Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring
title_full Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring
title_fullStr Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring
title_full_unstemmed Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring
title_short Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring
title_sort maternal high-fat diet associated with altered gene expression, dna methylation, and obesity risk in mouse offspring
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813940/
https://www.ncbi.nlm.nih.gov/pubmed/29447215
http://dx.doi.org/10.1371/journal.pone.0192606
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