Elevated HERV-K Expression in Soft Tissue Sarcoma Is Associated with Worsened Relapse-Free Survival

A wide variety of endogenous retroviral sequences has been demonstrated in the human genome so far, divided into several different families according to the sequence homology to viral strains. While increased expression of human endogenous retrovirus (HERV) elements has already been linked to unfavo...

Descripción completa

Detalles Bibliográficos
Autores principales: Giebler, Maria, Staege, Martin S., Blauschmidt, Sindy, Ohm, Lea I., Kraus, Matthias, Würl, Peter, Taubert, Helge, Greither, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5816752/
https://www.ncbi.nlm.nih.gov/pubmed/29487589
http://dx.doi.org/10.3389/fmicb.2018.00211
_version_ 1783300738406940672
author Giebler, Maria
Staege, Martin S.
Blauschmidt, Sindy
Ohm, Lea I.
Kraus, Matthias
Würl, Peter
Taubert, Helge
Greither, Thomas
author_facet Giebler, Maria
Staege, Martin S.
Blauschmidt, Sindy
Ohm, Lea I.
Kraus, Matthias
Würl, Peter
Taubert, Helge
Greither, Thomas
author_sort Giebler, Maria
collection PubMed
description A wide variety of endogenous retroviral sequences has been demonstrated in the human genome so far, divided into several different families according to the sequence homology to viral strains. While increased expression of human endogenous retrovirus (HERV) elements has already been linked to unfavorable prognosis in hepatocellular carcinoma, breast cancer, and ovarian carcinoma yet less is known about the impact of the expression of different HERV elements on sarcomagenesis in general as well as the outcome of soft tissue sarcoma (STS) patients. Therefore, in this study the association between expression of HERV-K and HERV-F and the clinicopathological characteristics in a cohort of STSs as well as the patients’ prognosis was evaluated. HERV-K and HERV-F expression was assessed by quantitative real-time PCR in 120 patient specimens. HERV-K and HERV-F expression was significantly correlated (r(S) = 0.5; p = 6.4 × 10(-9); Spearman’s rank bivariate correlation). Also, tumor diameter exhibited a significant negative association to HERV-K and HERV-F expression. Levels of several hypoxia-related RNAs like HIF-1α and miR-210 showed a significant positive correlation with both HERV-K and HERV-F expression. Although in survival analyses no impact of HERV expression on disease-specific survival could be detected, patients with elevated HERV-K expression had a significantly shorter relapse-free survival (p = 0.014, log-rank analysis). In conclusion, we provide evidence for the first time that the increased expression of HERV-K in tumors is associated with STS patients’ prognosis.
format Online
Article
Text
id pubmed-5816752
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-58167522018-02-27 Elevated HERV-K Expression in Soft Tissue Sarcoma Is Associated with Worsened Relapse-Free Survival Giebler, Maria Staege, Martin S. Blauschmidt, Sindy Ohm, Lea I. Kraus, Matthias Würl, Peter Taubert, Helge Greither, Thomas Front Microbiol Microbiology A wide variety of endogenous retroviral sequences has been demonstrated in the human genome so far, divided into several different families according to the sequence homology to viral strains. While increased expression of human endogenous retrovirus (HERV) elements has already been linked to unfavorable prognosis in hepatocellular carcinoma, breast cancer, and ovarian carcinoma yet less is known about the impact of the expression of different HERV elements on sarcomagenesis in general as well as the outcome of soft tissue sarcoma (STS) patients. Therefore, in this study the association between expression of HERV-K and HERV-F and the clinicopathological characteristics in a cohort of STSs as well as the patients’ prognosis was evaluated. HERV-K and HERV-F expression was assessed by quantitative real-time PCR in 120 patient specimens. HERV-K and HERV-F expression was significantly correlated (r(S) = 0.5; p = 6.4 × 10(-9); Spearman’s rank bivariate correlation). Also, tumor diameter exhibited a significant negative association to HERV-K and HERV-F expression. Levels of several hypoxia-related RNAs like HIF-1α and miR-210 showed a significant positive correlation with both HERV-K and HERV-F expression. Although in survival analyses no impact of HERV expression on disease-specific survival could be detected, patients with elevated HERV-K expression had a significantly shorter relapse-free survival (p = 0.014, log-rank analysis). In conclusion, we provide evidence for the first time that the increased expression of HERV-K in tumors is associated with STS patients’ prognosis. Frontiers Media S.A. 2018-02-13 /pmc/articles/PMC5816752/ /pubmed/29487589 http://dx.doi.org/10.3389/fmicb.2018.00211 Text en Copyright © 2018 Giebler, Staege, Blauschmidt, Ohm, Kraus, Würl, Taubert and Greither. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Giebler, Maria
Staege, Martin S.
Blauschmidt, Sindy
Ohm, Lea I.
Kraus, Matthias
Würl, Peter
Taubert, Helge
Greither, Thomas
Elevated HERV-K Expression in Soft Tissue Sarcoma Is Associated with Worsened Relapse-Free Survival
title Elevated HERV-K Expression in Soft Tissue Sarcoma Is Associated with Worsened Relapse-Free Survival
title_full Elevated HERV-K Expression in Soft Tissue Sarcoma Is Associated with Worsened Relapse-Free Survival
title_fullStr Elevated HERV-K Expression in Soft Tissue Sarcoma Is Associated with Worsened Relapse-Free Survival
title_full_unstemmed Elevated HERV-K Expression in Soft Tissue Sarcoma Is Associated with Worsened Relapse-Free Survival
title_short Elevated HERV-K Expression in Soft Tissue Sarcoma Is Associated with Worsened Relapse-Free Survival
title_sort elevated herv-k expression in soft tissue sarcoma is associated with worsened relapse-free survival
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5816752/
https://www.ncbi.nlm.nih.gov/pubmed/29487589
http://dx.doi.org/10.3389/fmicb.2018.00211
work_keys_str_mv AT gieblermaria elevatedhervkexpressioninsofttissuesarcomaisassociatedwithworsenedrelapsefreesurvival
AT staegemartins elevatedhervkexpressioninsofttissuesarcomaisassociatedwithworsenedrelapsefreesurvival
AT blauschmidtsindy elevatedhervkexpressioninsofttissuesarcomaisassociatedwithworsenedrelapsefreesurvival
AT ohmleai elevatedhervkexpressioninsofttissuesarcomaisassociatedwithworsenedrelapsefreesurvival
AT krausmatthias elevatedhervkexpressioninsofttissuesarcomaisassociatedwithworsenedrelapsefreesurvival
AT wurlpeter elevatedhervkexpressioninsofttissuesarcomaisassociatedwithworsenedrelapsefreesurvival
AT tauberthelge elevatedhervkexpressioninsofttissuesarcomaisassociatedwithworsenedrelapsefreesurvival
AT greitherthomas elevatedhervkexpressioninsofttissuesarcomaisassociatedwithworsenedrelapsefreesurvival