Profiling the transcriptome by RNA SPOTs

Single molecule FISH (smFISH) has been the gold standard in quantifying individual transcripts abundances. Here, we demonstrate the scaling up of smFISH to the transcriptome level by profiling of 10,212 different mRNAs from mouse fibroblast and embryonic stem cells. This methods, called RNA SPOTs (S...

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Detalles Bibliográficos
Autores principales: Eng, Chee-Huat Linus, Shah, Sheel, Thomassie, Julian, Cai, Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5819366/
https://www.ncbi.nlm.nih.gov/pubmed/29131163
http://dx.doi.org/10.1038/nmeth.4500

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5819366/
https://www.ncbi.nlm.nih.gov/pubmed/29131163
http://dx.doi.org/10.1038/nmeth.4500

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