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True gene-targeting events by CRISPR/Cas-induced DSB repair of the PPO locus with an ectopically integrated repair template

In recent years, several tools have become available for improved gene-targeting (GT) in plants. DNA breaks at specific sites activate local DNA repair and recombination, including recombination with ectopic sequences leading to GT. Large-scale transformation with the repair template can be avoided...

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Detalles Bibliográficos
Autores principales:	de Pater, Sylvia, Klemann, Bart J. P. M., Hooykaas, Paul J. J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5820285/ https://www.ncbi.nlm.nih.gov/pubmed/29463822 http://dx.doi.org/10.1038/s41598-018-21697-z

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