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Nobiletin Inhibits Hepatic Lipogenesis via Activation of AMP-Activated Protein Kinase
We aimed to investigate the effects of nobiletin on hepatic lipogenesis in high glucose-induced lipid accumulation in HepG2 cells. Nobiletin, a citrus polymethoxyflavonoid with six methoxy groups, is present abundantly in the peels of citrus fruits. HepG2 cells were incubated in Dulbecco's modi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5820556/ https://www.ncbi.nlm.nih.gov/pubmed/29552085 http://dx.doi.org/10.1155/2018/7420265 |
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author | Yuk, Taewon Kim, Younghwa Yang, Jinwoo Sung, Jeehye Jeong, Heon Sang Lee, Junsoo |
author_facet | Yuk, Taewon Kim, Younghwa Yang, Jinwoo Sung, Jeehye Jeong, Heon Sang Lee, Junsoo |
author_sort | Yuk, Taewon |
collection | PubMed |
description | We aimed to investigate the effects of nobiletin on hepatic lipogenesis in high glucose-induced lipid accumulation in HepG2 cells. Nobiletin, a citrus polymethoxyflavonoid with six methoxy groups, is present abundantly in the peels of citrus fruits. HepG2 cells were incubated in Dulbecco's modified Eagle's medium containing high glucose (25 mM) and subsequently treated with nobiletin at different concentrations (5, 25, and 50 μM). Results showed that nobiletin markedly inhibited high glucose-induced hepatic lipid accumulation in HepG2 cells. In addition, it reduced the protein expression of lipogenic factors, including sterol regulatory element-binding protein 1c (SREBP-1c) and fatty acid synthase (FAS). Nobiletin significantly increased the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase. Pretreatment with compound C, an AMPK inhibitor, abolished the inhibitory effects of nobiletin on SREBP-1c and FAS expression. These results suggested that nobiletin might attenuate high glucose-induced lipid accumulation in HepG2 hepatocytes via modulation of AMPK signaling pathway. Therefore, nobiletin might be useful for the prevention and treatment of nonalcoholic fatty liver diseases. |
format | Online Article Text |
id | pubmed-5820556 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-58205562018-03-18 Nobiletin Inhibits Hepatic Lipogenesis via Activation of AMP-Activated Protein Kinase Yuk, Taewon Kim, Younghwa Yang, Jinwoo Sung, Jeehye Jeong, Heon Sang Lee, Junsoo Evid Based Complement Alternat Med Research Article We aimed to investigate the effects of nobiletin on hepatic lipogenesis in high glucose-induced lipid accumulation in HepG2 cells. Nobiletin, a citrus polymethoxyflavonoid with six methoxy groups, is present abundantly in the peels of citrus fruits. HepG2 cells were incubated in Dulbecco's modified Eagle's medium containing high glucose (25 mM) and subsequently treated with nobiletin at different concentrations (5, 25, and 50 μM). Results showed that nobiletin markedly inhibited high glucose-induced hepatic lipid accumulation in HepG2 cells. In addition, it reduced the protein expression of lipogenic factors, including sterol regulatory element-binding protein 1c (SREBP-1c) and fatty acid synthase (FAS). Nobiletin significantly increased the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase. Pretreatment with compound C, an AMPK inhibitor, abolished the inhibitory effects of nobiletin on SREBP-1c and FAS expression. These results suggested that nobiletin might attenuate high glucose-induced lipid accumulation in HepG2 hepatocytes via modulation of AMPK signaling pathway. Therefore, nobiletin might be useful for the prevention and treatment of nonalcoholic fatty liver diseases. Hindawi 2018-02-07 /pmc/articles/PMC5820556/ /pubmed/29552085 http://dx.doi.org/10.1155/2018/7420265 Text en Copyright © 2018 Taewon Yuk et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yuk, Taewon Kim, Younghwa Yang, Jinwoo Sung, Jeehye Jeong, Heon Sang Lee, Junsoo Nobiletin Inhibits Hepatic Lipogenesis via Activation of AMP-Activated Protein Kinase |
title | Nobiletin Inhibits Hepatic Lipogenesis via Activation of AMP-Activated Protein Kinase |
title_full | Nobiletin Inhibits Hepatic Lipogenesis via Activation of AMP-Activated Protein Kinase |
title_fullStr | Nobiletin Inhibits Hepatic Lipogenesis via Activation of AMP-Activated Protein Kinase |
title_full_unstemmed | Nobiletin Inhibits Hepatic Lipogenesis via Activation of AMP-Activated Protein Kinase |
title_short | Nobiletin Inhibits Hepatic Lipogenesis via Activation of AMP-Activated Protein Kinase |
title_sort | nobiletin inhibits hepatic lipogenesis via activation of amp-activated protein kinase |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5820556/ https://www.ncbi.nlm.nih.gov/pubmed/29552085 http://dx.doi.org/10.1155/2018/7420265 |
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