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The Essential Genome of Escherichia coli K-12

Transposon-directed insertion site sequencing (TraDIS) is a high-throughput method coupling transposon mutagenesis with short-fragment DNA sequencing. It is commonly used to identify essential genes. Single gene deletion libraries are considered the gold standard for identifying essential genes. Cur...

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Detalles Bibliográficos
Autores principales: Goodall, Emily C. A., Robinson, Ashley, Johnston, Iain G., Jabbari, Sara, Turner, Keith A., Cunningham, Adam F., Lund, Peter A., Cole, Jeffrey A., Henderson, Ian R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5821084/
https://www.ncbi.nlm.nih.gov/pubmed/29463657
http://dx.doi.org/10.1128/mBio.02096-17