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Direct DNA and RNA detection from large volumes of whole human blood

PCR inhibitors in clinical specimens negatively affect the sensitivity of diagnostic PCR and RT-PCR or may even cause false-negative results. To overcome PCR inhibition, increase the sensitivity of the assays and simplify the detection protocols, simple methods based on quantitative nested real-time...

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Detalles Bibliográficos
Autores principales: Cai, Dongyang, Behrmann, Ole, Hufert, Frank, Dame, Gregory, Urban, Gerald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5821888/
https://www.ncbi.nlm.nih.gov/pubmed/29467420
http://dx.doi.org/10.1038/s41598-018-21224-0