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Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system

Protein degradation is a fundamental process in all living cells and is essential to remove both damaged proteins and intact proteins that are no longer needed by the cell. We are interested in creating synthetic genetic circuits that function in a cell-free expression system. This will require not...

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Detalles Bibliográficos
Autores principales: Shi, Xinying, Wu, Ti, M. Cole, Christian, K. Devaraj, Neal, Joseph, Simpson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823941/
https://www.ncbi.nlm.nih.gov/pubmed/29472573
http://dx.doi.org/10.1038/s41598-018-21739-6