miPrimer: an empirical-based qPCR primer design method for small noncoding microRNA

MicroRNAs (miRNAs) are 18–25 nucleotides (nt) of highly conserved, noncoding RNAs involved in gene regulation. Because of miRNAs’ short length, the design of miRNA primers for PCR amplification remains a significant challenge. Adding to the challenge are miRNAs similar in sequence and miRNA family m...

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Detalles Bibliográficos
Autores principales: Kang, Shih-Ting, Hsieh, Yi-Shan, Feng, Chi-Ting, Chen, Yu-Ting, Yang, Pok Eric, Chen, Wei-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Bioinformatics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824350/
https://www.ncbi.nlm.nih.gov/pubmed/29208706
http://dx.doi.org/10.1261/rna.061150.117

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824350/
https://www.ncbi.nlm.nih.gov/pubmed/29208706
http://dx.doi.org/10.1261/rna.061150.117

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