Cargando…

miPrimer: an empirical-based qPCR primer design method for small noncoding microRNA

MicroRNAs (miRNAs) are 18–25 nucleotides (nt) of highly conserved, noncoding RNAs involved in gene regulation. Because of miRNAs’ short length, the design of miRNA primers for PCR amplification remains a significant challenge. Adding to the challenge are miRNAs similar in sequence and miRNA family m...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kang, Shih-Ting, Hsieh, Yi-Shan, Feng, Chi-Ting, Chen, Yu-Ting, Yang, Pok Eric, Chen, Wei-Ming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2018
Materias:	Bioinformatics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824350/ https://www.ncbi.nlm.nih.gov/pubmed/29208706 http://dx.doi.org/10.1261/rna.061150.117

Ejemplares similares

SpeciesPrimer: a bioinformatics pipeline dedicated to the design of qPCR primers for the quantification of bacterial species
por: Dreier, Matthias, et al.
Publicado: (2020)

Linear-hairpin variable primer RT-qPCR for MicroRNA
por: Lan, Lin, et al.
Publicado: (2018)

qPCR primer design revisited
por: Bustin, Stephen, et al.
Publicado: (2017)

A tool for design of primers for microRNA-specific quantitative RT-qPCR
por: Busk, Peter K
Publicado: (2014)

Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping
por: Li, Baowei, et al.
Publicado: (2021)

qPrimerDB: a thermodynamics-based gene-specific qPCR primer database for 147 organisms
por: Lu, Kun, et al.
Publicado: (2018)

pcr: an R package for quality assessment, analysis and testing of qPCR data
por: Ahmed, Mahmoud, et al.
Publicado: (2018)

Design and Assessment of Species-Level qPCR Primers Targeting Comammox
por: Beach, Natalie K., et al.
Publicado: (2019)

CIRCprimerXL: Convenient and High-Throughput PCR Primer Design for Circular RNA Quantification
por: Vromman, Marieke, et al.
Publicado: (2022)

RT-qPCR Testing of SARS-CoV-2: A Primer
por: Bustin, Stephen A., et al.
Publicado: (2020)

Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells
por: Chen, Gang, et al.
Publicado: (2010)

GPrimer: a fast GPU-based pipeline for primer design for qPCR experiments
por: Bae, Jeongmin, et al.
Publicado: (2021)

Innovative microRNA quantification by qPCR
por: Le, Minh Ngoc, et al.
Publicado: (2023)

PhyloPrimer: a taxon-specific oligonucleotide design platform
por: Varliero, Gilda, et al.
Publicado: (2021)

OUHP: an optimized universal hairpin primer system for cost-effective and high-throughput RT-qPCR-based quantification of microRNA (miRNA) expression
por: He, Fang, et al.
Publicado: (2021)

qPrimerDepot: a primer database for quantitative real time PCR
por: Cui, Wenwu, et al.
Publicado: (2007)

The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing
por: Herbert, Mike, et al.
Publicado: (2011)

GETPrime 2.0: gene- and transcript-specific qPCR primers for 13 species including polymorphisms
por: David, Fabrice P.A., et al.
Publicado: (2017)

Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil
por: Arenas, Francisco, et al.
Publicado: (2022)

Improved Efficiency and Robustness in qPCR and Multiplex End-Point PCR by Twisted Intercalating Nucleic Acid Modified Primers
por: Schneider, Uffe Vest, et al.
Publicado: (2012)

Superior Multiplexing Capacity of PlexPrimers Enables Sensitive and Specific Detection of SNPs and Clustered Mutations in qPCR
por: Tan, Lit Yeen, et al.
Publicado: (2017)

High Functional Stability of a Low-cost HBV DNA qPCR Primer Pair and Plasmid Standard
por: Aguiar, Jorge, et al.
Publicado: (2016)

ChIPprimersDB: a public repository of verified qPCR primers for chromatin immunoprecipitation (ChIP)
por: Kurtenbach, Stefan, et al.
Publicado: (2018)

Data in support of qPCR primer design and verification in a Pink1 −/− rat model of Parkinson disease
por: Kelm-Nelson, Cynthia A., et al.
Publicado: (2016)

Lyophilized Matrix Containing Ready-to-Use Primers and Probe Solution for Standardization of Real-Time PCR and RT-qPCR Diagnostics in Virology
por: Thirion, Laurence, et al.
Publicado: (2020)

MRPrimerW: a tool for rapid design of valid high-quality primers for multiple target qPCR experiments
por: Kim, Hyerin, et al.
Publicado: (2016)

1786. An Automated Method to Assess Oligonucleotide Primer and Probe Complementarity to Genomic Targets in Infectious Disease qPCR Assays
por: Sinha, Rohita, et al.
Publicado: (2019)

In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR
por: Zimmermann, Fee, et al.
Publicado: (2022)

A Novel Helper qPCR Assay for the Detection of miRNA Using Target/Helper Template for Primer Formation
por: Jiang, Xianfeng, et al.
Publicado: (2022)

Design of a data set of qPCR primers for the early region of Human Papillomavirus oncogenic types 16 and 18
por: Kantún-Moreno, Nuvia, et al.
Publicado: (2023)

The role of RNA structure at 5′ untranslated region in microRNA-mediated gene regulation
por: Gu, Wanjun, et al.
Publicado: (2014)

Development of a Novel Primer–TaqMan Probe Set for Diagnosis and Quantification of Meloidogyne enterolobii in Soil Using qPCR and Droplet Digital PCR Assays
por: Chen, Yuan, et al.
Publicado: (2022)

Primer set 2.0 for highly parallel qPCR array targeting antibiotic resistance genes and mobile genetic elements
por: Stedtfeld, Robert D, et al.
Publicado: (2018)

Analytical performance comparison of four SARS-CoV-2 RT-qPCR primer-probe sets for wastewater samples
por: Zhang, Shuxin, et al.
Publicado: (2022)

Optimal RNA isolation method and primer design to detect gene knockdown by qPCR when validating Drosophila transgenic RNAi lines
por: Mainland, Roslyn L., et al.
Publicado: (2017)

MRPrimerW2: an enhanced tool for rapid design of valid high-quality primers with multiple search modes for qPCR experiments
por: Jeon, Hajin, et al.
Publicado: (2019)

Single well, single-common primer pair, dual probe, duplex qPCR assay for the quantification of mRNA splicing variants
por: Wang, Janice, et al.
Publicado: (2021)

MorphoCatcher: a multiple-alignment based web tool for target selection and designing taxon-specific primers in the loop-mediated isothermal amplification method
por: Shirshikov, Fedor V., et al.
Publicado: (2019)

Adapterama I: universal stubs and primers for 384 unique dual-indexed or 147,456 combinatorially-indexed Illumina libraries (iTru & iNext)
por: Glenn, Travis C., et al.
Publicado: (2019)

Compared with conventional PCR assay, qPCR assay greatly improves the detection efficiency of predation
por: Yang, Ting‐bang, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_h776jdumf0f73eqi2h4gbcpg77