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Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegars and molecular analyses

BACKGROUND AND OBJECTIVES: Acetic acid bacteria (AAB) are one of the major interests of researchers. Traditional vinegars are suitable sources of AAB because they are not undergone industrial process like filtering and adding preservatives. Komagataeibacter xylinus as a member of AAB is known as the...

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Autores principales: Lavasani, Paria Sadat, Motevaseli, Elahe, Shirzad, Mahdieh, Modarressi, Mohammad Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5825934/
https://www.ncbi.nlm.nih.gov/pubmed/29487732
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author Lavasani, Paria Sadat
Motevaseli, Elahe
Shirzad, Mahdieh
Modarressi, Mohammad Hossein
author_facet Lavasani, Paria Sadat
Motevaseli, Elahe
Shirzad, Mahdieh
Modarressi, Mohammad Hossein
author_sort Lavasani, Paria Sadat
collection PubMed
description BACKGROUND AND OBJECTIVES: Acetic acid bacteria (AAB) are one of the major interests of researchers. Traditional vinegars are suitable sources of AAB because they are not undergone industrial process like filtering and adding preservatives. Komagataeibacter xylinus as a member of AAB is known as the main cellulose producer among other bacteria. The purpose of the current study was to isolate the bacteria from traditional vinegars and its molecular analyses. MATERIALS AND METHODS: Vinegar samples were collected. Well-organized bacteriological tests were carried out to differentiate isolated bacteria from other cellulose producers and to identify K. xylinus. NaOH treatment and Calcofluor white staining were used for detecting cellulose. Chromosomal DNA of each strain was extracted via three methods of boiling, phenol-chloroform and sonication. Molecular analyses were performed on the basis of 16S rRNA sequences and cellulose synthase catalytic subunit gene (bcsA) for further confirmation. Phylogenetic tree was constructed for more characterization. Two housekeeping genes were studied including phenylalanyl-tRNA synthase alpha subunit (pheS) and RNA polymerase alpha subunit (rpoA). RESULTS: Of the 97 samples, 43 K. xylinus strains were isolated. They were identified via bacteriological and molecular techniques. 16S rDNA sequence showed 99% similarity with registered sequences of the bacteria. Biodiversity of the genome confirmed by analyzing bcsA, pheS and rpoA genes. CONCLUSION: K. xylinus can be isolated from traditional vinegars. Screening tests ought to include the classical methods and molecular techniques. Different molecular techniques and more genomic research should be developed to expand our knowledge for distinguishing isolated bacteria especially in the fields of AAB.
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spelling pubmed-58259342018-02-27 Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegars and molecular analyses Lavasani, Paria Sadat Motevaseli, Elahe Shirzad, Mahdieh Modarressi, Mohammad Hossein Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Acetic acid bacteria (AAB) are one of the major interests of researchers. Traditional vinegars are suitable sources of AAB because they are not undergone industrial process like filtering and adding preservatives. Komagataeibacter xylinus as a member of AAB is known as the main cellulose producer among other bacteria. The purpose of the current study was to isolate the bacteria from traditional vinegars and its molecular analyses. MATERIALS AND METHODS: Vinegar samples were collected. Well-organized bacteriological tests were carried out to differentiate isolated bacteria from other cellulose producers and to identify K. xylinus. NaOH treatment and Calcofluor white staining were used for detecting cellulose. Chromosomal DNA of each strain was extracted via three methods of boiling, phenol-chloroform and sonication. Molecular analyses were performed on the basis of 16S rRNA sequences and cellulose synthase catalytic subunit gene (bcsA) for further confirmation. Phylogenetic tree was constructed for more characterization. Two housekeeping genes were studied including phenylalanyl-tRNA synthase alpha subunit (pheS) and RNA polymerase alpha subunit (rpoA). RESULTS: Of the 97 samples, 43 K. xylinus strains were isolated. They were identified via bacteriological and molecular techniques. 16S rDNA sequence showed 99% similarity with registered sequences of the bacteria. Biodiversity of the genome confirmed by analyzing bcsA, pheS and rpoA genes. CONCLUSION: K. xylinus can be isolated from traditional vinegars. Screening tests ought to include the classical methods and molecular techniques. Different molecular techniques and more genomic research should be developed to expand our knowledge for distinguishing isolated bacteria especially in the fields of AAB. Tehran University of Medical Sciences 2017-12 /pmc/articles/PMC5825934/ /pubmed/29487732 Text en Copyright© 2017 Iranian Neuroscience Society http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lavasani, Paria Sadat
Motevaseli, Elahe
Shirzad, Mahdieh
Modarressi, Mohammad Hossein
Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegars and molecular analyses
title Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegars and molecular analyses
title_full Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegars and molecular analyses
title_fullStr Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegars and molecular analyses
title_full_unstemmed Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegars and molecular analyses
title_short Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegars and molecular analyses
title_sort isolation and identification of komagataeibacter xylinus from iranian traditional vinegars and molecular analyses
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5825934/
https://www.ncbi.nlm.nih.gov/pubmed/29487732
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