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Extreme heterogeneity of influenza virus infection in single cells

Viral infection can dramatically alter a cell’s transcriptome. However, these changes have mostly been studied by bulk measurements on many cells. Here we use single-cell mRNA sequencing to examine the transcriptional consequences of influenza virus infection. We find extremely wide cell-to-cell var...

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Detalles Bibliográficos
Autores principales: Russell, Alistair B, Trapnell, Cole, Bloom, Jesse D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5826275/
https://www.ncbi.nlm.nih.gov/pubmed/29451492
http://dx.doi.org/10.7554/eLife.32303
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author Russell, Alistair B
Trapnell, Cole
Bloom, Jesse D
author_facet Russell, Alistair B
Trapnell, Cole
Bloom, Jesse D
author_sort Russell, Alistair B
collection PubMed
description Viral infection can dramatically alter a cell’s transcriptome. However, these changes have mostly been studied by bulk measurements on many cells. Here we use single-cell mRNA sequencing to examine the transcriptional consequences of influenza virus infection. We find extremely wide cell-to-cell variation in the productivity of viral transcription – viral transcripts comprise less than a percent of total mRNA in many infected cells, but a few cells derive over half their mRNA from virus. Some infected cells fail to express at least one viral gene, but this gene absence only partially explains variation in viral transcriptional load. Despite variation in viral load, the relative abundances of viral mRNAs are fairly consistent across infected cells. Activation of innate immune pathways is rare, but some cellular genes co-vary in abundance with the amount of viral mRNA. Overall, our results highlight the complexity of viral infection at the level of single cells.
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spelling pubmed-58262752018-02-28 Extreme heterogeneity of influenza virus infection in single cells Russell, Alistair B Trapnell, Cole Bloom, Jesse D eLife Microbiology and Infectious Disease Viral infection can dramatically alter a cell’s transcriptome. However, these changes have mostly been studied by bulk measurements on many cells. Here we use single-cell mRNA sequencing to examine the transcriptional consequences of influenza virus infection. We find extremely wide cell-to-cell variation in the productivity of viral transcription – viral transcripts comprise less than a percent of total mRNA in many infected cells, but a few cells derive over half their mRNA from virus. Some infected cells fail to express at least one viral gene, but this gene absence only partially explains variation in viral transcriptional load. Despite variation in viral load, the relative abundances of viral mRNAs are fairly consistent across infected cells. Activation of innate immune pathways is rare, but some cellular genes co-vary in abundance with the amount of viral mRNA. Overall, our results highlight the complexity of viral infection at the level of single cells. eLife Sciences Publications, Ltd 2018-02-16 /pmc/articles/PMC5826275/ /pubmed/29451492 http://dx.doi.org/10.7554/eLife.32303 Text en © 2018, Russell et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Russell, Alistair B
Trapnell, Cole
Bloom, Jesse D
Extreme heterogeneity of influenza virus infection in single cells
title Extreme heterogeneity of influenza virus infection in single cells
title_full Extreme heterogeneity of influenza virus infection in single cells
title_fullStr Extreme heterogeneity of influenza virus infection in single cells
title_full_unstemmed Extreme heterogeneity of influenza virus infection in single cells
title_short Extreme heterogeneity of influenza virus infection in single cells
title_sort extreme heterogeneity of influenza virus infection in single cells
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5826275/
https://www.ncbi.nlm.nih.gov/pubmed/29451492
http://dx.doi.org/10.7554/eLife.32303
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