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Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors

Arginine-vasopressin (AVP) neurons exist in the hypothalamus, a major region of the diencephalon, and play an essential role in water balance. Here, we established the differentiation method for AVP-secreting neurons from human embryonic stem cells (hESCs) by recapitulating in vitro the in vivo embr...

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Autores principales: Ogawa, Koichiro, Suga, Hidetaka, Ozone, Chikafumi, Sakakibara, Mayu, Yamada, Tomiko, Kano, Mayuko, Mitsumoto, Kazuki, Kasai, Takatoshi, Kodani, Yu, Nagasaki, Hiroshi, Yamamoto, Naoki, Hagiwara, Daisuke, Goto, Motomitsu, Banno, Ryoichi, Sugimura, Yoshihisa, Arima, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827757/
https://www.ncbi.nlm.nih.gov/pubmed/29483626
http://dx.doi.org/10.1038/s41598-018-22053-x
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author Ogawa, Koichiro
Suga, Hidetaka
Ozone, Chikafumi
Sakakibara, Mayu
Yamada, Tomiko
Kano, Mayuko
Mitsumoto, Kazuki
Kasai, Takatoshi
Kodani, Yu
Nagasaki, Hiroshi
Yamamoto, Naoki
Hagiwara, Daisuke
Goto, Motomitsu
Banno, Ryoichi
Sugimura, Yoshihisa
Arima, Hiroshi
author_facet Ogawa, Koichiro
Suga, Hidetaka
Ozone, Chikafumi
Sakakibara, Mayu
Yamada, Tomiko
Kano, Mayuko
Mitsumoto, Kazuki
Kasai, Takatoshi
Kodani, Yu
Nagasaki, Hiroshi
Yamamoto, Naoki
Hagiwara, Daisuke
Goto, Motomitsu
Banno, Ryoichi
Sugimura, Yoshihisa
Arima, Hiroshi
author_sort Ogawa, Koichiro
collection PubMed
description Arginine-vasopressin (AVP) neurons exist in the hypothalamus, a major region of the diencephalon, and play an essential role in water balance. Here, we established the differentiation method for AVP-secreting neurons from human embryonic stem cells (hESCs) by recapitulating in vitro the in vivo embryonic developmental processes of AVP neurons. At first, the differentiation efficiency was improved. That was achieved through the optimization of the culture condition for obtaining dorsal hypothalamic progenitors. Secondly, the induced AVP neurons were identified by immunohistochemistry and these neurons secreted AVP after potassium chloride stimulation. Additionally, other hypothalamic neuropeptides were also detected, such as oxytocin, corticotropin-releasing hormone, thyrotropin-releasing hormone, pro-opiomelanocortin, agouti-related peptide, orexin, and melanin-concentrating hormone. This is the first report describing the generation of secretory AVP neurons derived from hESCs. This method will be applicable to research using disease models and, potentially, for regenerative medicine of the hypothalamus.
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spelling pubmed-58277572018-03-01 Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors Ogawa, Koichiro Suga, Hidetaka Ozone, Chikafumi Sakakibara, Mayu Yamada, Tomiko Kano, Mayuko Mitsumoto, Kazuki Kasai, Takatoshi Kodani, Yu Nagasaki, Hiroshi Yamamoto, Naoki Hagiwara, Daisuke Goto, Motomitsu Banno, Ryoichi Sugimura, Yoshihisa Arima, Hiroshi Sci Rep Article Arginine-vasopressin (AVP) neurons exist in the hypothalamus, a major region of the diencephalon, and play an essential role in water balance. Here, we established the differentiation method for AVP-secreting neurons from human embryonic stem cells (hESCs) by recapitulating in vitro the in vivo embryonic developmental processes of AVP neurons. At first, the differentiation efficiency was improved. That was achieved through the optimization of the culture condition for obtaining dorsal hypothalamic progenitors. Secondly, the induced AVP neurons were identified by immunohistochemistry and these neurons secreted AVP after potassium chloride stimulation. Additionally, other hypothalamic neuropeptides were also detected, such as oxytocin, corticotropin-releasing hormone, thyrotropin-releasing hormone, pro-opiomelanocortin, agouti-related peptide, orexin, and melanin-concentrating hormone. This is the first report describing the generation of secretory AVP neurons derived from hESCs. This method will be applicable to research using disease models and, potentially, for regenerative medicine of the hypothalamus. Nature Publishing Group UK 2018-02-26 /pmc/articles/PMC5827757/ /pubmed/29483626 http://dx.doi.org/10.1038/s41598-018-22053-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ogawa, Koichiro
Suga, Hidetaka
Ozone, Chikafumi
Sakakibara, Mayu
Yamada, Tomiko
Kano, Mayuko
Mitsumoto, Kazuki
Kasai, Takatoshi
Kodani, Yu
Nagasaki, Hiroshi
Yamamoto, Naoki
Hagiwara, Daisuke
Goto, Motomitsu
Banno, Ryoichi
Sugimura, Yoshihisa
Arima, Hiroshi
Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
title Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
title_full Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
title_fullStr Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
title_full_unstemmed Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
title_short Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
title_sort vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827757/
https://www.ncbi.nlm.nih.gov/pubmed/29483626
http://dx.doi.org/10.1038/s41598-018-22053-x
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