Extraction of total RNA from single-oocytes and single-cell mRNA sequencing of swine oocytes

OBJECTIVE: Analyses of single oocytes are essential for a fine dissection of molecular features governing developmental competence. We adapted the phenol–chloroform procedure for the purification of total RNA from single oocytes. RESULTS: Key modifications include the use of Phasemaker™ tubes, a sec...

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Detalles Bibliográficos
Autores principales: Kimble, Katelyn M., Dickinson, Sarah E., Biase, Fernando H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828076/
https://www.ncbi.nlm.nih.gov/pubmed/29482623
http://dx.doi.org/10.1186/s13104-018-3264-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828076/
https://www.ncbi.nlm.nih.gov/pubmed/29482623
http://dx.doi.org/10.1186/s13104-018-3264-2

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