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Extraction of total RNA from single-oocytes and single-cell mRNA sequencing of swine oocytes
OBJECTIVE: Analyses of single oocytes are essential for a fine dissection of molecular features governing developmental competence. We adapted the phenol–chloroform procedure for the purification of total RNA from single oocytes. RESULTS: Key modifications include the use of Phasemaker™ tubes, a sec...
Autores principales: | Kimble, Katelyn M., Dickinson, Sarah E., Biase, Fernando H. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828076/ https://www.ncbi.nlm.nih.gov/pubmed/29482623 http://dx.doi.org/10.1186/s13104-018-3264-2 |
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