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Aggregate Size Dependence of Amyloid Adsorption onto Charged Interfaces
[Image: see text] Amyloid aggregates are associated with a range of human neurodegenerative disorders, and it has been shown that neurotoxicity is dependent on aggregate size. Combining molecular simulation with analytical theory, a predictive model is proposed for the adsorption of amyloid aggregat...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828364/ https://www.ncbi.nlm.nih.gov/pubmed/29284092 http://dx.doi.org/10.1021/acs.langmuir.7b03155 |
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author | Tesei, Giulio Hellstrand, Erik Sanagavarapu, Kalyani Linse, Sara Sparr, Emma Vácha, Robert Lund, Mikael |
author_facet | Tesei, Giulio Hellstrand, Erik Sanagavarapu, Kalyani Linse, Sara Sparr, Emma Vácha, Robert Lund, Mikael |
author_sort | Tesei, Giulio |
collection | PubMed |
description | [Image: see text] Amyloid aggregates are associated with a range of human neurodegenerative disorders, and it has been shown that neurotoxicity is dependent on aggregate size. Combining molecular simulation with analytical theory, a predictive model is proposed for the adsorption of amyloid aggregates onto oppositely charged surfaces, where the interaction is governed by an interplay between electrostatic attraction and entropic repulsion. Predictions are experimentally validated against quartz crystal microbalance–dissipation experiments of amyloid beta peptides and fragmented fibrils in the presence of a supported lipid bilayer. Assuming amyloids as rigid, elongated particles, we observe nonmonotonic trends for the extent of adsorption with respect to aggregate size and preferential adsorption of smaller aggregates over larger ones. Our findings describe a general phenomenon with implications for stiff polyions and rodlike particles that are electrostatically attracted to a surface. |
format | Online Article Text |
id | pubmed-5828364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American
Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-58283642018-02-28 Aggregate Size Dependence of Amyloid Adsorption onto Charged Interfaces Tesei, Giulio Hellstrand, Erik Sanagavarapu, Kalyani Linse, Sara Sparr, Emma Vácha, Robert Lund, Mikael Langmuir [Image: see text] Amyloid aggregates are associated with a range of human neurodegenerative disorders, and it has been shown that neurotoxicity is dependent on aggregate size. Combining molecular simulation with analytical theory, a predictive model is proposed for the adsorption of amyloid aggregates onto oppositely charged surfaces, where the interaction is governed by an interplay between electrostatic attraction and entropic repulsion. Predictions are experimentally validated against quartz crystal microbalance–dissipation experiments of amyloid beta peptides and fragmented fibrils in the presence of a supported lipid bilayer. Assuming amyloids as rigid, elongated particles, we observe nonmonotonic trends for the extent of adsorption with respect to aggregate size and preferential adsorption of smaller aggregates over larger ones. Our findings describe a general phenomenon with implications for stiff polyions and rodlike particles that are electrostatically attracted to a surface. American Chemical Society 2017-12-28 2018-01-30 /pmc/articles/PMC5828364/ /pubmed/29284092 http://dx.doi.org/10.1021/acs.langmuir.7b03155 Text en Copyright © 2017 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Tesei, Giulio Hellstrand, Erik Sanagavarapu, Kalyani Linse, Sara Sparr, Emma Vácha, Robert Lund, Mikael Aggregate Size Dependence of Amyloid Adsorption onto Charged Interfaces |
title | Aggregate Size Dependence of Amyloid Adsorption onto
Charged Interfaces |
title_full | Aggregate Size Dependence of Amyloid Adsorption onto
Charged Interfaces |
title_fullStr | Aggregate Size Dependence of Amyloid Adsorption onto
Charged Interfaces |
title_full_unstemmed | Aggregate Size Dependence of Amyloid Adsorption onto
Charged Interfaces |
title_short | Aggregate Size Dependence of Amyloid Adsorption onto
Charged Interfaces |
title_sort | aggregate size dependence of amyloid adsorption onto
charged interfaces |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828364/ https://www.ncbi.nlm.nih.gov/pubmed/29284092 http://dx.doi.org/10.1021/acs.langmuir.7b03155 |
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