Synergistic effect of mutagenesis and truncation to improve a polyesterase from Clostridium botulinum for polyester hydrolysis

The activity of the esterase (Cbotu_EstA) from Clostridium botulinum on the polyester poly(ethylene terephthalate) (PET) was improved by concomitant engineering of two different domains. On the one hand, the zinc-binding domain present in Cbotu_EstA was subjected to site-directed mutagenesis. On the...

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Detalles Bibliográficos
Autores principales: Biundo, Antonino, Reich, Johanna, Ribitsch, Doris, Guebitz, Georg M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829244/
https://www.ncbi.nlm.nih.gov/pubmed/29487314
http://dx.doi.org/10.1038/s41598-018-21825-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829244/
https://www.ncbi.nlm.nih.gov/pubmed/29487314
http://dx.doi.org/10.1038/s41598-018-21825-9

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