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Efficient in situ barcode sequencing using padlock probe-based BaristaSeq

Cellular DNA/RNA tags (barcodes) allow for multiplexed cell lineage tracing and neuronal projection mapping with cellular resolution. Conventional approaches to reading out cellular barcodes trade off spatial resolution with throughput. Bulk sequencing achieves high throughput but sacrifices spatial...

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Detalles Bibliográficos
Autores principales: Chen, Xiaoyin, Sun, Yu-Chi, Church, George M, Lee, Je Hyuk, Zador, Anthony M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829746/
https://www.ncbi.nlm.nih.gov/pubmed/29190363
http://dx.doi.org/10.1093/nar/gkx1206