Cargando…

Transplanted Donor- or Stem Cell-Derived Cone Photoreceptors Can Both Integrate and Undergo Material Transfer in an Environment-Dependent Manner

Human vision relies heavily upon cone photoreceptors, and their loss results in permanent visual impairment. Transplantation of healthy photoreceptors can restore visual function in models of inherited blindness, a process previously understood to arise by donor cell integration within the host reti...

Descripción completa

Detalles Bibliográficos
Autores principales: Waldron, Paul V., Di Marco, Fabiana, Kruczek, Kamil, Ribeiro, Joana, Graca, Anna B., Hippert, Claire, Aghaizu, Nozie D., Kalargyrou, Aikaterini A., Barber, Amanda C., Grimaldi, Giulia, Duran, Yanai, Blackford, Samuel J.I., Kloc, Magdalena, Goh, Debbie, Zabala Aldunate, Eduardo, Sampson, Robert D., Bainbridge, James W.B., Smith, Alexander J., Gonzalez-Cordero, Anai, Sowden, Jane C., Ali, Robin R., Pearson, Rachael A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5830910/
https://www.ncbi.nlm.nih.gov/pubmed/29307580
http://dx.doi.org/10.1016/j.stemcr.2017.12.008
_version_ 1783303087505539072
author Waldron, Paul V.
Di Marco, Fabiana
Kruczek, Kamil
Ribeiro, Joana
Graca, Anna B.
Hippert, Claire
Aghaizu, Nozie D.
Kalargyrou, Aikaterini A.
Barber, Amanda C.
Grimaldi, Giulia
Duran, Yanai
Blackford, Samuel J.I.
Kloc, Magdalena
Goh, Debbie
Zabala Aldunate, Eduardo
Sampson, Robert D.
Bainbridge, James W.B.
Smith, Alexander J.
Gonzalez-Cordero, Anai
Sowden, Jane C.
Ali, Robin R.
Pearson, Rachael A.
author_facet Waldron, Paul V.
Di Marco, Fabiana
Kruczek, Kamil
Ribeiro, Joana
Graca, Anna B.
Hippert, Claire
Aghaizu, Nozie D.
Kalargyrou, Aikaterini A.
Barber, Amanda C.
Grimaldi, Giulia
Duran, Yanai
Blackford, Samuel J.I.
Kloc, Magdalena
Goh, Debbie
Zabala Aldunate, Eduardo
Sampson, Robert D.
Bainbridge, James W.B.
Smith, Alexander J.
Gonzalez-Cordero, Anai
Sowden, Jane C.
Ali, Robin R.
Pearson, Rachael A.
author_sort Waldron, Paul V.
collection PubMed
description Human vision relies heavily upon cone photoreceptors, and their loss results in permanent visual impairment. Transplantation of healthy photoreceptors can restore visual function in models of inherited blindness, a process previously understood to arise by donor cell integration within the host retina. However, we and others recently demonstrated that donor rod photoreceptors engage in material transfer with host photoreceptors, leading to the host cells acquiring proteins otherwise expressed only by donor cells. We sought to determine whether stem cell- and donor-derived cones undergo integration and/or material transfer. We find that material transfer accounts for a significant proportion of rescued cells following cone transplantation into non-degenerative hosts. Strikingly, however, substantial numbers of cones integrated into the Nrl(−/−) and Prph2(rd2/rd2), but not Nrl(−/−);RPE65(R91W/R91W), murine models of retinal degeneration. This confirms the occurrence of photoreceptor integration in certain models of retinal degeneration and demonstrates the importance of the host environment in determining transplantation outcome.
format Online
Article
Text
id pubmed-5830910
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-58309102018-03-06 Transplanted Donor- or Stem Cell-Derived Cone Photoreceptors Can Both Integrate and Undergo Material Transfer in an Environment-Dependent Manner Waldron, Paul V. Di Marco, Fabiana Kruczek, Kamil Ribeiro, Joana Graca, Anna B. Hippert, Claire Aghaizu, Nozie D. Kalargyrou, Aikaterini A. Barber, Amanda C. Grimaldi, Giulia Duran, Yanai Blackford, Samuel J.I. Kloc, Magdalena Goh, Debbie Zabala Aldunate, Eduardo Sampson, Robert D. Bainbridge, James W.B. Smith, Alexander J. Gonzalez-Cordero, Anai Sowden, Jane C. Ali, Robin R. Pearson, Rachael A. Stem Cell Reports Article Human vision relies heavily upon cone photoreceptors, and their loss results in permanent visual impairment. Transplantation of healthy photoreceptors can restore visual function in models of inherited blindness, a process previously understood to arise by donor cell integration within the host retina. However, we and others recently demonstrated that donor rod photoreceptors engage in material transfer with host photoreceptors, leading to the host cells acquiring proteins otherwise expressed only by donor cells. We sought to determine whether stem cell- and donor-derived cones undergo integration and/or material transfer. We find that material transfer accounts for a significant proportion of rescued cells following cone transplantation into non-degenerative hosts. Strikingly, however, substantial numbers of cones integrated into the Nrl(−/−) and Prph2(rd2/rd2), but not Nrl(−/−);RPE65(R91W/R91W), murine models of retinal degeneration. This confirms the occurrence of photoreceptor integration in certain models of retinal degeneration and demonstrates the importance of the host environment in determining transplantation outcome. Elsevier 2018-01-04 /pmc/articles/PMC5830910/ /pubmed/29307580 http://dx.doi.org/10.1016/j.stemcr.2017.12.008 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Waldron, Paul V.
Di Marco, Fabiana
Kruczek, Kamil
Ribeiro, Joana
Graca, Anna B.
Hippert, Claire
Aghaizu, Nozie D.
Kalargyrou, Aikaterini A.
Barber, Amanda C.
Grimaldi, Giulia
Duran, Yanai
Blackford, Samuel J.I.
Kloc, Magdalena
Goh, Debbie
Zabala Aldunate, Eduardo
Sampson, Robert D.
Bainbridge, James W.B.
Smith, Alexander J.
Gonzalez-Cordero, Anai
Sowden, Jane C.
Ali, Robin R.
Pearson, Rachael A.
Transplanted Donor- or Stem Cell-Derived Cone Photoreceptors Can Both Integrate and Undergo Material Transfer in an Environment-Dependent Manner
title Transplanted Donor- or Stem Cell-Derived Cone Photoreceptors Can Both Integrate and Undergo Material Transfer in an Environment-Dependent Manner
title_full Transplanted Donor- or Stem Cell-Derived Cone Photoreceptors Can Both Integrate and Undergo Material Transfer in an Environment-Dependent Manner
title_fullStr Transplanted Donor- or Stem Cell-Derived Cone Photoreceptors Can Both Integrate and Undergo Material Transfer in an Environment-Dependent Manner
title_full_unstemmed Transplanted Donor- or Stem Cell-Derived Cone Photoreceptors Can Both Integrate and Undergo Material Transfer in an Environment-Dependent Manner
title_short Transplanted Donor- or Stem Cell-Derived Cone Photoreceptors Can Both Integrate and Undergo Material Transfer in an Environment-Dependent Manner
title_sort transplanted donor- or stem cell-derived cone photoreceptors can both integrate and undergo material transfer in an environment-dependent manner
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5830910/
https://www.ncbi.nlm.nih.gov/pubmed/29307580
http://dx.doi.org/10.1016/j.stemcr.2017.12.008
work_keys_str_mv AT waldronpaulv transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT dimarcofabiana transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT kruczekkamil transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT ribeirojoana transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT gracaannab transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT hippertclaire transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT aghaizunozied transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT kalargyrouaikaterinia transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT barberamandac transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT grimaldigiulia transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT duranyanai transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT blackfordsamuelji transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT klocmagdalena transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT gohdebbie transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT zabalaaldunateeduardo transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT sampsonrobertd transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT bainbridgejameswb transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT smithalexanderj transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT gonzalezcorderoanai transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT sowdenjanec transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT alirobinr transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner
AT pearsonrachaela transplanteddonororstemcellderivedconephotoreceptorscanbothintegrateandundergomaterialtransferinanenvironmentdependentmanner