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XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer
Pig cloning by somatic cell nuclear transfer (SCNT) remains extremely inefficient, and many cloned embryos undergo abnormal development. Here, by profiling transcriptome expression, we observed dysregulated chromosome-wide gene expression in every chromosome and identified a considerable number of g...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5830944/ https://www.ncbi.nlm.nih.gov/pubmed/29337117 http://dx.doi.org/10.1016/j.stemcr.2017.12.015 |
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author | Ruan, Degong Peng, Jiangyun Wang, Xiaoshan Ouyang, Zhen Zou, Qingjian Yang, Yi Chen, Fangbing Ge, Weikai Wu, Han Liu, Zhaoming Zhao, Yu Zhao, Bentian Zhang, Quanjun Lai, Chengdan Fan, Nana Zhou, Zhiwei Liu, Qishuai Li, Nan Jin, Qin Shi, Hui Xie, Jingke Song, Hong Yang, Xiaoyu Chen, Jiekai Wang, Kepin Li, Xiaoping Lai, Liangxue |
author_facet | Ruan, Degong Peng, Jiangyun Wang, Xiaoshan Ouyang, Zhen Zou, Qingjian Yang, Yi Chen, Fangbing Ge, Weikai Wu, Han Liu, Zhaoming Zhao, Yu Zhao, Bentian Zhang, Quanjun Lai, Chengdan Fan, Nana Zhou, Zhiwei Liu, Qishuai Li, Nan Jin, Qin Shi, Hui Xie, Jingke Song, Hong Yang, Xiaoyu Chen, Jiekai Wang, Kepin Li, Xiaoping Lai, Liangxue |
author_sort | Ruan, Degong |
collection | PubMed |
description | Pig cloning by somatic cell nuclear transfer (SCNT) remains extremely inefficient, and many cloned embryos undergo abnormal development. Here, by profiling transcriptome expression, we observed dysregulated chromosome-wide gene expression in every chromosome and identified a considerable number of genes that are aberrantly expressed in the abnormal cloned embryos. In particular, XIST, a long non-coding RNA gene, showed high ectopic expression in abnormal embryos. We also proved that nullification of the XIST gene in donor cells can normalize aberrant gene expression in cloned embryos and enhance long-term development capacity of the embryos. Furthermore, the increased quality of XIST-deficient embryos was associated with the global H3K9me3 reduction. Injection of H3K9me demethylase Kdm4A into NT embryos could improve the development of pre-implantation stage embryos. However, Kdm4A addition also induced XIST derepression in the active X chromosome and thus was not able to enhance the in vivo long-term developmental capacity of porcine NT embryos. |
format | Online Article Text |
id | pubmed-5830944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-58309442018-03-06 XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer Ruan, Degong Peng, Jiangyun Wang, Xiaoshan Ouyang, Zhen Zou, Qingjian Yang, Yi Chen, Fangbing Ge, Weikai Wu, Han Liu, Zhaoming Zhao, Yu Zhao, Bentian Zhang, Quanjun Lai, Chengdan Fan, Nana Zhou, Zhiwei Liu, Qishuai Li, Nan Jin, Qin Shi, Hui Xie, Jingke Song, Hong Yang, Xiaoyu Chen, Jiekai Wang, Kepin Li, Xiaoping Lai, Liangxue Stem Cell Reports Article Pig cloning by somatic cell nuclear transfer (SCNT) remains extremely inefficient, and many cloned embryos undergo abnormal development. Here, by profiling transcriptome expression, we observed dysregulated chromosome-wide gene expression in every chromosome and identified a considerable number of genes that are aberrantly expressed in the abnormal cloned embryos. In particular, XIST, a long non-coding RNA gene, showed high ectopic expression in abnormal embryos. We also proved that nullification of the XIST gene in donor cells can normalize aberrant gene expression in cloned embryos and enhance long-term development capacity of the embryos. Furthermore, the increased quality of XIST-deficient embryos was associated with the global H3K9me3 reduction. Injection of H3K9me demethylase Kdm4A into NT embryos could improve the development of pre-implantation stage embryos. However, Kdm4A addition also induced XIST derepression in the active X chromosome and thus was not able to enhance the in vivo long-term developmental capacity of porcine NT embryos. Elsevier 2018-01-11 /pmc/articles/PMC5830944/ /pubmed/29337117 http://dx.doi.org/10.1016/j.stemcr.2017.12.015 Text en © 2017 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Ruan, Degong Peng, Jiangyun Wang, Xiaoshan Ouyang, Zhen Zou, Qingjian Yang, Yi Chen, Fangbing Ge, Weikai Wu, Han Liu, Zhaoming Zhao, Yu Zhao, Bentian Zhang, Quanjun Lai, Chengdan Fan, Nana Zhou, Zhiwei Liu, Qishuai Li, Nan Jin, Qin Shi, Hui Xie, Jingke Song, Hong Yang, Xiaoyu Chen, Jiekai Wang, Kepin Li, Xiaoping Lai, Liangxue XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer |
title | XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer |
title_full | XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer |
title_fullStr | XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer |
title_full_unstemmed | XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer |
title_short | XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer |
title_sort | xist derepression in active x chromosome hinders pig somatic cell nuclear transfer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5830944/ https://www.ncbi.nlm.nih.gov/pubmed/29337117 http://dx.doi.org/10.1016/j.stemcr.2017.12.015 |
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