TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding

RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, a chemical method that uses oxidative-nucleophilic-aromatic-substitution to convert 4-thiouridine into cytidine analogues, yielding apparent U-t...

Descripción completa

Detalles Bibliográficos
Autores principales: Schofield, Jeremy A., Duffy, Erin E., Kiefer, Lea, Sullivan, Meaghan C., Simon, Matthew D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831505/
https://www.ncbi.nlm.nih.gov/pubmed/29355846
http://dx.doi.org/10.1038/nmeth.4582

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831505/
https://www.ncbi.nlm.nih.gov/pubmed/29355846
http://dx.doi.org/10.1038/nmeth.4582

Ejemplares similares