TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding

RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, a chemical method that uses oxidative-nucleophilic-aromatic-substitution to convert 4-thiouridine into cytidine analogues, yielding apparent U-t...

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Detalles Bibliográficos
Autores principales: Schofield, Jeremy A., Duffy, Erin E., Kiefer, Lea, Sullivan, Meaghan C., Simon, Matthew D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831505/
https://www.ncbi.nlm.nih.gov/pubmed/29355846
http://dx.doi.org/10.1038/nmeth.4582
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author Schofield, Jeremy A.
Duffy, Erin E.
Kiefer, Lea
Sullivan, Meaghan C.
Simon, Matthew D.
author_facet Schofield, Jeremy A.
Duffy, Erin E.
Kiefer, Lea
Sullivan, Meaghan C.
Simon, Matthew D.
author_sort Schofield, Jeremy A.
collection PubMed
description RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, a chemical method that uses oxidative-nucleophilic-aromatic-substitution to convert 4-thiouridine into cytidine analogues, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single molecule approach that is adaptable to many applications, and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq.
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spelling pubmed-58315052018-07-22 TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding Schofield, Jeremy A. Duffy, Erin E. Kiefer, Lea Sullivan, Meaghan C. Simon, Matthew D. Nat Methods Article RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, a chemical method that uses oxidative-nucleophilic-aromatic-substitution to convert 4-thiouridine into cytidine analogues, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single molecule approach that is adaptable to many applications, and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq. 2018-01-22 2018-03 /pmc/articles/PMC5831505/ /pubmed/29355846 http://dx.doi.org/10.1038/nmeth.4582 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Schofield, Jeremy A.
Duffy, Erin E.
Kiefer, Lea
Sullivan, Meaghan C.
Simon, Matthew D.
TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding
title TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding
title_full TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding
title_fullStr TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding
title_full_unstemmed TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding
title_short TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding
title_sort timelapse-seq: adding a temporal dimension to rna sequencing through nucleoside recoding
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831505/
https://www.ncbi.nlm.nih.gov/pubmed/29355846
http://dx.doi.org/10.1038/nmeth.4582
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