Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology

The generation of genetically modified alleles in mice using conventional transgenesis technologies is a long and inefficient process. A new study shows that the in situ delivery of CRISPR/Cas9 reagents into pregnant mice results in a high efficiency of editing, and enables the rapid generation of b...

Descripción completa

Detalles Bibliográficos
Autor principal: Burgio, Gaetan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Highlight
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831610/
https://www.ncbi.nlm.nih.gov/pubmed/29490686
http://dx.doi.org/10.1186/s13059-018-1409-1
_version_ 1783303171577217024
author Burgio, Gaetan
author_facet Burgio, Gaetan
author_sort Burgio, Gaetan
collection PubMed
description The generation of genetically modified alleles in mice using conventional transgenesis technologies is a long and inefficient process. A new study shows that the in situ delivery of CRISPR/Cas9 reagents into pregnant mice results in a high efficiency of editing, and enables the rapid generation of both simple and complex alleles.
format Online
Article
Text
id pubmed-5831610
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-58316102018-03-05 Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology Burgio, Gaetan Genome Biol Research Highlight The generation of genetically modified alleles in mice using conventional transgenesis technologies is a long and inefficient process. A new study shows that the in situ delivery of CRISPR/Cas9 reagents into pregnant mice results in a high efficiency of editing, and enables the rapid generation of both simple and complex alleles. BioMed Central 2018-02-28 /pmc/articles/PMC5831610/ /pubmed/29490686 http://dx.doi.org/10.1186/s13059-018-1409-1 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Highlight
Burgio, Gaetan
Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology
title Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology
title_full Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology
title_fullStr Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology
title_full_unstemmed Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology
title_short Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology
title_sort redefining mouse transgenesis with crispr/cas9 genome editing technology
topic Research Highlight
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831610/
https://www.ncbi.nlm.nih.gov/pubmed/29490686
http://dx.doi.org/10.1186/s13059-018-1409-1
work_keys_str_mv AT burgiogaetan redefiningmousetransgenesiswithcrisprcas9genomeeditingtechnology

Ejemplares similares