A 340/380 nm light‐emitting diode illuminator for Fura‐2 AM ratiometric Ca(2+) imaging of live cells with better than 5 nM precision

We report the first demonstration of a fast wavelength‐switchable 340/380 nm light‐emitting diode (LED) illuminator for Fura‐2 ratiometric Ca(2+) imaging of live cells. The LEDs closely match the excitation peaks of bound and free Fura‐2 and enables the precise detection of cytosolic Ca(2+) concentr...

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Detalles Bibliográficos
Autores principales: TINNING, P.W., FRANSSEN, A.J.P.M., HRIDI, S.U., BUSHELL, T.J., MCCONNELL, G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5836901/
https://www.ncbi.nlm.nih.gov/pubmed/28837217
http://dx.doi.org/10.1111/jmi.12616

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5836901/
https://www.ncbi.nlm.nih.gov/pubmed/28837217
http://dx.doi.org/10.1111/jmi.12616

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