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A 340/380 nm light‐emitting diode illuminator for Fura‐2 AM ratiometric Ca(2+) imaging of live cells with better than 5 nM precision
We report the first demonstration of a fast wavelength‐switchable 340/380 nm light‐emitting diode (LED) illuminator for Fura‐2 ratiometric Ca(2+) imaging of live cells. The LEDs closely match the excitation peaks of bound and free Fura‐2 and enables the precise detection of cytosolic Ca(2+) concentr...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5836901/ https://www.ncbi.nlm.nih.gov/pubmed/28837217 http://dx.doi.org/10.1111/jmi.12616 |