Cargando…

Microhomology-assisted scarless genome editing in human iPSCs

Gene-edited induced pluripotent stem cells (iPSCs) provide relevant isogenic human disease models in patient-specific or healthy genetic backgrounds. Towards this end, gene targeting using antibiotic selection along with engineered point mutations remains a reliable method to enrich edited cells. Ne...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kim, Shin-Il, Matsumoto, Tomoko, Kagawa, Harunobu, Nakamura, Michiko, Hirohata, Ryoko, Ueno, Ayano, Ohishi, Maki, Sakuma, Tetsushi, Soga, Tomoyoshi, Yamamoto, Takashi, Woltjen, Knut
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5838097/ https://www.ncbi.nlm.nih.gov/pubmed/29507284 http://dx.doi.org/10.1038/s41467-018-03044-y

Ejemplares similares

OVOL1 Influences the Determination and Expansion of iPSC Reprogramming Intermediates
por: Kagawa, Harunobu, et al.
Publicado: (2019)

Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations
por: Grajcarek, Janin, et al.
Publicado: (2019)

N-Terminal Amino Acids Determine KLF4 Protein Stability in 2A Peptide-Linked Polycistronic Reprogramming Constructs
por: Reinhardt, Anika, et al.
Publicado: (2020)

One-Step Biallelic and Scarless Correction of a β-Thalassemia Mutation in Patient-Specific iPSCs without Drug Selection
por: Liu, Yali, et al.
Publicado: (2017)

Lucky iPSCs
por: Zviran, Asaf, et al.
Publicado: (2014)

iPSCs are safe!
por: Yan, Hualong, et al.
Publicado: (2017)

Platforms of in vivo genome editing with inducible Cas9 for advanced cancer modeling
por: Jo, Norihide, et al.
Publicado: (2019)

Improved and Flexible HDR Editing by Targeting Introns in iPSCs
por: Fu, Juan, et al.
Publicado: (2022)

Targeted Integration of Inducible Caspase-9 in Human iPSCs Allows Efficient in vitro Clearance of iPSCs and iPSC-Macrophages
por: Lipus, Alexandra, et al.
Publicado: (2020)

Mitochondria fingerprint longevity in iPSCs
por: Masotti, Andrea, et al.
Publicado: (2015)

Applications of iPSCs in Cancer Research
por: Kim, Jean J
Publicado: (2015)

Reducing genomic instability in iPSCs
por: Ruiz, Sergio, et al.
Publicado: (2015)

The Challenge of Bringing iPSCs to the Patient
por: Ortuño-Costela, María del Carmen, et al.
Publicado: (2019)

iPSCs in Modeling and Therapy of Osteoarthritis
por: Csobonyeiova, Maria, et al.
Publicado: (2021)

Microhomology Selection for Microhomology Mediated End Joining in Saccharomyces cerevisiae
por: Lee, Kihoon, et al.
Publicado: (2019)

iPSCs: From Bench to Clinical Bed
por: Li, Yujing, et al.
Publicado: (2016)

Differentiation of human iPSCs into functional podocytes
por: Rauch, Caroline, et al.
Publicado: (2018)

Human iPSCs and Genome Editing Technologies for Precision Cardiovascular Tissue Engineering
por: Gähwiler, Eric K. N., et al.
Publicado: (2021)

Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes
por: Tanigawa, Shunsuke, et al.
Publicado: (2018)

Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection
por: Mitzelfelt, Katie A., et al.
Publicado: (2017)

Nanog-Independent Reprogramming to iPSCs with Canonical Factors
por: Carter, Ava C., et al.
Publicado: (2014)

Disease, iPSCs and a Quest for the Perfect Model
Publicado: (2016)

Clinical Therapy Using iPSCs: Hopes and Challenges
por: Lu, Xiao, et al.
Publicado: (2013)

The Potential of iPSCs for the Treatment of Premature Aging Disorders
por: Compagnucci, Claudia, et al.
Publicado: (2017)

The tale of autologous iPSCs: A monkey perspective
por: Li, Fei
Publicado: (2014)

Tumorigenicity risk of iPSCs in vivo: nip it in the bud
por: Zhong, Chaoliang, et al.
Publicado: (2022)

Functional genomics and the future of iPSCs in disease modeling
por: Brooks, Imogen R., et al.
Publicado: (2022)

Modelling urea cycle disorders using iPSCs
por: Duff, Claire, et al.
Publicado: (2022)

iPSCs in NK Cell Manufacturing and NKEV Development
por: Boyd-Gibbins, Nicholas, et al.
Publicado: (2022)

MSCs vs. iPSCs: Potential in therapeutic applications
por: Thanaskody, Kalaiselvaan, et al.
Publicado: (2022)

Identifying the dynamics of actin and tubulin polymerization in iPSCs and in iPSC-derived neurons
por: Magliocca, Valentina, et al.
Publicado: (2017)

CDK8/19 inhibition plays an important role in pancreatic β-cell induction from human iPSCs
por: Sakuma, Kensuke, et al.
Publicado: (2023)

Deficiency in MT5-MMP Supports Branching of Human iPSCs-Derived Neurons and Reduces Expression of GLAST/S100 in iPSCs-Derived Astrocytes
por: Arnst, Nikita, et al.
Publicado: (2021)

 Potential of Gene Editing and Induced Pluripotent Stem Cells (iPSCs) in Treatment of Retinal Diseases 
por: Chuang, Katherine, et al.
Publicado: (2017)

Modelling DMD-associated cardiomyopathy in patient-derived iPSCs
Publicado: (2015)

The State of Play with iPSCs and Spinal Cord Injury Models
por: Hodgetts, Stuart I., et al.
Publicado: (2015)

An Overview of Direct Somatic Reprogramming: The Ins and Outs of iPSCs
por: Menon, Siddharth, et al.
Publicado: (2016)

Epigenetic Modifiers Facilitate Induction and Pluripotency of Porcine iPSCs
por: Mao, Jian, et al.
Publicado: (2016)

Apoptosis in Porcine Pluripotent Cells: From ICM to iPSCs
por: Kim, Eunhye, et al.
Publicado: (2016)

The rebirth of iPSCs: Towards a healthier epigenetic landscape
por: Li, Fei
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_dajmobq77ov9193chc7givimrm