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Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells
CRISPR (clustered, regularly interspaced, short palindromic repeats)/Cas9 (CRISPR-associated protein 9) has become the tool of choice for generating gene knockouts across a variety of species. The ability for efficient gene editing in primary T cells not only represents a valuable research tool to s...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Rockefeller University Press
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5839763/ https://www.ncbi.nlm.nih.gov/pubmed/29436394 http://dx.doi.org/10.1084/jem.20171626 |