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Comparative Evaluation of Three Preprocessing Methods for Extraction and Detection of Influenza A Virus Nucleic Acids from Sputum

Viscous sputum specimens usually cannot undergo automated extraction, and thus, a pre-homogenization process is desirable before isolating nucleic acids for real-time reverse transcription PCR. In this study, we compared three preprocessing methods [preprocessing with normal saline (NS), dithiothrei...

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Detalles Bibliográficos
Autores principales: Yu, Fei, Qiu, Ting, Zeng, Ying, Wang, Yiyin, Zheng, Shufa, Chen, Xiao, Chen, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840144/
https://www.ncbi.nlm.nih.gov/pubmed/29552562
http://dx.doi.org/10.3389/fmed.2018.00056