Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes

Over the past few years, tools that make use of the Cas9 nuclease have led to many breakthroughs, including in the control of gene expression. The catalytically dead variant of Cas9 known as dCas9 can be guided by small RNAs to block transcription of target genes, in a strategy also known as CRISPRi...

Descripción completa

Detalles Bibliográficos
Autores principales: Vigouroux, Antoine, Oldewurtel, Enno, Cui, Lun, Bikard, David, van Teeffelen, Sven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5842579/
https://www.ncbi.nlm.nih.gov/pubmed/29519933
http://dx.doi.org/10.15252/msb.20177899

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5842579/
https://www.ncbi.nlm.nih.gov/pubmed/29519933
http://dx.doi.org/10.15252/msb.20177899

Ejemplares similares