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Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads

High-throughput single-cell RNA-seq methods assign limited unique molecular identifier (UMI) counts as gene expression values to single cells from shallow sequence reads and detect limited gene counts. We thus developed a high-throughput single-cell RNA-seq method, Quartz-Seq2, to overcome these iss...

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Detalles Bibliográficos
Autores principales: Sasagawa, Yohei, Danno, Hiroki, Takada, Hitomi, Ebisawa, Masashi, Tanaka, Kaori, Hayashi, Tetsutaro, Kurisaki, Akira, Nikaido, Itoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845169/
https://www.ncbi.nlm.nih.gov/pubmed/29523163
http://dx.doi.org/10.1186/s13059-018-1407-3