Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390

The Staphylococcus aureus type VII protein secretion system (T7SS) plays important roles in virulence and intra-species competition. Here we show that the T7SS in strain RN6390 is activated by supplementing the growth medium with haemoglobin, and its cofactor haemin (haem B). Transcript analysis and...

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Autores principales: Casabona, M. Guillermina, Kneuper, Holger, Alferes de Lima, Daniela, Harkins, Catriona P., Zoltner, Martin, Hjerde, Erik, Holden, Matthew T. G., Palmer, Tracy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845736/
https://www.ncbi.nlm.nih.gov/pubmed/29171824
http://dx.doi.org/10.1099/mic.0.000579
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author Casabona, M. Guillermina
Kneuper, Holger
Alferes de Lima, Daniela
Harkins, Catriona P.
Zoltner, Martin
Hjerde, Erik
Holden, Matthew T. G.
Palmer, Tracy
author_facet Casabona, M. Guillermina
Kneuper, Holger
Alferes de Lima, Daniela
Harkins, Catriona P.
Zoltner, Martin
Hjerde, Erik
Holden, Matthew T. G.
Palmer, Tracy
author_sort Casabona, M. Guillermina
collection PubMed
description The Staphylococcus aureus type VII protein secretion system (T7SS) plays important roles in virulence and intra-species competition. Here we show that the T7SS in strain RN6390 is activated by supplementing the growth medium with haemoglobin, and its cofactor haemin (haem B). Transcript analysis and secretion assays suggest that activation by haemin occurs at a transcriptional and a post-translational level. Loss of T7 secretion activity by deletion of essC results in upregulation of genes required for iron acquisition. Taken together these findings suggest that the T7SS plays a role in iron homeostasis in at least some S. aureus strains.
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spelling pubmed-58457362018-03-13 Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390 Casabona, M. Guillermina Kneuper, Holger Alferes de Lima, Daniela Harkins, Catriona P. Zoltner, Martin Hjerde, Erik Holden, Matthew T. G. Palmer, Tracy Microbiology (Reading) Research Article The Staphylococcus aureus type VII protein secretion system (T7SS) plays important roles in virulence and intra-species competition. Here we show that the T7SS in strain RN6390 is activated by supplementing the growth medium with haemoglobin, and its cofactor haemin (haem B). Transcript analysis and secretion assays suggest that activation by haemin occurs at a transcriptional and a post-translational level. Loss of T7 secretion activity by deletion of essC results in upregulation of genes required for iron acquisition. Taken together these findings suggest that the T7SS plays a role in iron homeostasis in at least some S. aureus strains. Microbiology Society 2017-12 2017-11-24 /pmc/articles/PMC5845736/ /pubmed/29171824 http://dx.doi.org/10.1099/mic.0.000579 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Casabona, M. Guillermina
Kneuper, Holger
Alferes de Lima, Daniela
Harkins, Catriona P.
Zoltner, Martin
Hjerde, Erik
Holden, Matthew T. G.
Palmer, Tracy
Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390
title Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390
title_full Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390
title_fullStr Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390
title_full_unstemmed Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390
title_short Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390
title_sort haem-iron plays a key role in the regulation of the ess/type vii secretion system of staphylococcus aureus rn6390
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845736/
https://www.ncbi.nlm.nih.gov/pubmed/29171824
http://dx.doi.org/10.1099/mic.0.000579
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