Cargando…

Modulation of Phase Shift between Wnt and Notch Signaling Oscillations Controls Mesoderm Segmentation

How signaling dynamics encode information is a central question in biology. During vertebrate development, dynamic Notch signaling oscillations control segmentation of the presomitic mesoderm (PSM). In mouse embryos, this molecular clock comprises signaling oscillations of several pathways, i.e., No...

Descripción completa

Detalles Bibliográficos
Autores principales: Sonnen, Katharina F., Lauschke, Volker M., Uraji, Julia, Falk, Henning J., Petersen, Yvonne, Funk, Maja C., Beaupeux, Mathias, François, Paul, Merten, Christoph A., Aulehla, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847172/
https://www.ncbi.nlm.nih.gov/pubmed/29474908
http://dx.doi.org/10.1016/j.cell.2018.01.026
_version_ 1783305701362237440
author Sonnen, Katharina F.
Lauschke, Volker M.
Uraji, Julia
Falk, Henning J.
Petersen, Yvonne
Funk, Maja C.
Beaupeux, Mathias
François, Paul
Merten, Christoph A.
Aulehla, Alexander
author_facet Sonnen, Katharina F.
Lauschke, Volker M.
Uraji, Julia
Falk, Henning J.
Petersen, Yvonne
Funk, Maja C.
Beaupeux, Mathias
François, Paul
Merten, Christoph A.
Aulehla, Alexander
author_sort Sonnen, Katharina F.
collection PubMed
description How signaling dynamics encode information is a central question in biology. During vertebrate development, dynamic Notch signaling oscillations control segmentation of the presomitic mesoderm (PSM). In mouse embryos, this molecular clock comprises signaling oscillations of several pathways, i.e., Notch, Wnt, and FGF signaling. Here, we directly address the role of the relative timing between Wnt and Notch signaling oscillations during PSM patterning. To this end, we developed a new experimental strategy using microfluidics-based entrainment that enables specific control of the rhythm of segmentation clock oscillations. Using this approach, we find that Wnt and Notch signaling are coupled at the level of their oscillation dynamics. Furthermore, we provide functional evidence that the oscillation phase shift between Wnt and Notch signaling is critical for PSM segmentation. Our work hence reveals that dynamic signaling, i.e., the relative timing between oscillatory signals, encodes essential information during multicellular development.
format Online
Article
Text
id pubmed-5847172
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Cell Press
record_format MEDLINE/PubMed
spelling pubmed-58471722018-03-13 Modulation of Phase Shift between Wnt and Notch Signaling Oscillations Controls Mesoderm Segmentation Sonnen, Katharina F. Lauschke, Volker M. Uraji, Julia Falk, Henning J. Petersen, Yvonne Funk, Maja C. Beaupeux, Mathias François, Paul Merten, Christoph A. Aulehla, Alexander Cell Article How signaling dynamics encode information is a central question in biology. During vertebrate development, dynamic Notch signaling oscillations control segmentation of the presomitic mesoderm (PSM). In mouse embryos, this molecular clock comprises signaling oscillations of several pathways, i.e., Notch, Wnt, and FGF signaling. Here, we directly address the role of the relative timing between Wnt and Notch signaling oscillations during PSM patterning. To this end, we developed a new experimental strategy using microfluidics-based entrainment that enables specific control of the rhythm of segmentation clock oscillations. Using this approach, we find that Wnt and Notch signaling are coupled at the level of their oscillation dynamics. Furthermore, we provide functional evidence that the oscillation phase shift between Wnt and Notch signaling is critical for PSM segmentation. Our work hence reveals that dynamic signaling, i.e., the relative timing between oscillatory signals, encodes essential information during multicellular development. Cell Press 2018-02-22 /pmc/articles/PMC5847172/ /pubmed/29474908 http://dx.doi.org/10.1016/j.cell.2018.01.026 Text en © 2018 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Sonnen, Katharina F.
Lauschke, Volker M.
Uraji, Julia
Falk, Henning J.
Petersen, Yvonne
Funk, Maja C.
Beaupeux, Mathias
François, Paul
Merten, Christoph A.
Aulehla, Alexander
Modulation of Phase Shift between Wnt and Notch Signaling Oscillations Controls Mesoderm Segmentation
title Modulation of Phase Shift between Wnt and Notch Signaling Oscillations Controls Mesoderm Segmentation
title_full Modulation of Phase Shift between Wnt and Notch Signaling Oscillations Controls Mesoderm Segmentation
title_fullStr Modulation of Phase Shift between Wnt and Notch Signaling Oscillations Controls Mesoderm Segmentation
title_full_unstemmed Modulation of Phase Shift between Wnt and Notch Signaling Oscillations Controls Mesoderm Segmentation
title_short Modulation of Phase Shift between Wnt and Notch Signaling Oscillations Controls Mesoderm Segmentation
title_sort modulation of phase shift between wnt and notch signaling oscillations controls mesoderm segmentation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847172/
https://www.ncbi.nlm.nih.gov/pubmed/29474908
http://dx.doi.org/10.1016/j.cell.2018.01.026
work_keys_str_mv AT sonnenkatharinaf modulationofphaseshiftbetweenwntandnotchsignalingoscillationscontrolsmesodermsegmentation
AT lauschkevolkerm modulationofphaseshiftbetweenwntandnotchsignalingoscillationscontrolsmesodermsegmentation
AT urajijulia modulationofphaseshiftbetweenwntandnotchsignalingoscillationscontrolsmesodermsegmentation
AT falkhenningj modulationofphaseshiftbetweenwntandnotchsignalingoscillationscontrolsmesodermsegmentation
AT petersenyvonne modulationofphaseshiftbetweenwntandnotchsignalingoscillationscontrolsmesodermsegmentation
AT funkmajac modulationofphaseshiftbetweenwntandnotchsignalingoscillationscontrolsmesodermsegmentation
AT beaupeuxmathias modulationofphaseshiftbetweenwntandnotchsignalingoscillationscontrolsmesodermsegmentation
AT francoispaul modulationofphaseshiftbetweenwntandnotchsignalingoscillationscontrolsmesodermsegmentation
AT mertenchristopha modulationofphaseshiftbetweenwntandnotchsignalingoscillationscontrolsmesodermsegmentation
AT aulehlaalexander modulationofphaseshiftbetweenwntandnotchsignalingoscillationscontrolsmesodermsegmentation