Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform

Directed evolution has long been a key strategy to generate enzymes with desired properties like high selectivity, but experimental barriers and analytical costs of screening enormous mutant libraries have limited such efforts. Here, we describe an ultrahigh-throughput dual-channel microfluidic drop...

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Detalles Bibliográficos
Autores principales: Ma, Fuqiang, Chung, Meng Ting, Yao, Yuan, Nidetz, Robert, Lee, Lap Man, Liu, Allen P., Feng, Yan, Kurabayashi, Katsuo, Yang, Guang-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847605/
https://www.ncbi.nlm.nih.gov/pubmed/29531246
http://dx.doi.org/10.1038/s41467-018-03492-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847605/
https://www.ncbi.nlm.nih.gov/pubmed/29531246
http://dx.doi.org/10.1038/s41467-018-03492-6

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