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Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
Directed evolution has long been a key strategy to generate enzymes with desired properties like high selectivity, but experimental barriers and analytical costs of screening enormous mutant libraries have limited such efforts. Here, we describe an ultrahigh-throughput dual-channel microfluidic drop...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847605/ https://www.ncbi.nlm.nih.gov/pubmed/29531246 http://dx.doi.org/10.1038/s41467-018-03492-6 |