Development of an Effective 6-Methylpurine Counterselection Marker for Genetic Manipulation in Thermococcus barophilus

A gene disruption system for Thermococcus barophilus was developed using simvastatin (HMG-CoA reductase encoding gene) for positive selection and 5-Fluoroorotic acid (5-FOA), a pyrF gene for negative selection. Multiple gene mutants were constructed with this system, which offers the possibility of...

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Detalles Bibliográficos
Autores principales: Birien, Tiphaine, Thiel, Axel, Henneke, Ghislaine, Flament, Didier, Moalic, Yann, Jebbar, Mohamed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852573/
https://www.ncbi.nlm.nih.gov/pubmed/29414865
http://dx.doi.org/10.3390/genes9020077

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852573/
https://www.ncbi.nlm.nih.gov/pubmed/29414865
http://dx.doi.org/10.3390/genes9020077

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