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Mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output
AIMS: A single isolated cardiomyocyte is the smallest functional unit of the heart. Yet, all single isolated cardiomyocyte experiments have been limited by the lack of proper methods that could reproduce a physiological cardiac cycle. We aimed to investigate the contractile properties of a single ca...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853507/ https://www.ncbi.nlm.nih.gov/pubmed/27037258 http://dx.doi.org/10.1093/cvr/cvw072 |
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author | Helmes, Michiel Najafi, Aref Palmer, Bradley M. Breel, Ernst Rijnveld, Niek Iannuzzi, Davide van der Velden, Jolanda |
author_facet | Helmes, Michiel Najafi, Aref Palmer, Bradley M. Breel, Ernst Rijnveld, Niek Iannuzzi, Davide van der Velden, Jolanda |
author_sort | Helmes, Michiel |
collection | PubMed |
description | AIMS: A single isolated cardiomyocyte is the smallest functional unit of the heart. Yet, all single isolated cardiomyocyte experiments have been limited by the lack of proper methods that could reproduce a physiological cardiac cycle. We aimed to investigate the contractile properties of a single cardiomyocyte that correctly mimic the cardiac cycle. METHODS AND RESULTS: By adjusting the parameters of the feedback loop, using a suitably engineered feedback system and recording the developed force and the length of a single rat cardiomyocyte during contraction and relaxation, we were able to construct force–length (FL) relations analogous to the pressure–volume (PV) relations at the whole heart level. From the cardiac loop graphs, we obtained, for the first time, the power generated by one single cardiomyocyte. CONCLUSION: Here, we introduce a new approach that by combining mechanics, electronics, and a new type optical force transducer can measure the FL relationship of a single isolated cardiomyocyte undergoing a mechanical loop that mimics the PV cycle of a beating heart. |
format | Online Article Text |
id | pubmed-5853507 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-58535072018-03-23 Mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output Helmes, Michiel Najafi, Aref Palmer, Bradley M. Breel, Ernst Rijnveld, Niek Iannuzzi, Davide van der Velden, Jolanda Cardiovasc Res Original Articles AIMS: A single isolated cardiomyocyte is the smallest functional unit of the heart. Yet, all single isolated cardiomyocyte experiments have been limited by the lack of proper methods that could reproduce a physiological cardiac cycle. We aimed to investigate the contractile properties of a single cardiomyocyte that correctly mimic the cardiac cycle. METHODS AND RESULTS: By adjusting the parameters of the feedback loop, using a suitably engineered feedback system and recording the developed force and the length of a single rat cardiomyocyte during contraction and relaxation, we were able to construct force–length (FL) relations analogous to the pressure–volume (PV) relations at the whole heart level. From the cardiac loop graphs, we obtained, for the first time, the power generated by one single cardiomyocyte. CONCLUSION: Here, we introduce a new approach that by combining mechanics, electronics, and a new type optical force transducer can measure the FL relationship of a single isolated cardiomyocyte undergoing a mechanical loop that mimics the PV cycle of a beating heart. Oxford University Press 2016-07-01 2016-04-01 /pmc/articles/PMC5853507/ /pubmed/27037258 http://dx.doi.org/10.1093/cvr/cvw072 Text en The Author 2016. Published by Oxford University Press on behalf of the European Society of Cardiology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Original Articles Helmes, Michiel Najafi, Aref Palmer, Bradley M. Breel, Ernst Rijnveld, Niek Iannuzzi, Davide van der Velden, Jolanda Mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output |
title | Mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output |
title_full | Mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output |
title_fullStr | Mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output |
title_full_unstemmed | Mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output |
title_short | Mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output |
title_sort | mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853507/ https://www.ncbi.nlm.nih.gov/pubmed/27037258 http://dx.doi.org/10.1093/cvr/cvw072 |
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