A universal vector concept for a direct genotyping of transgenic organisms and a systematic creation of homozygous lines

Diploid transgenic organisms are either hemi- or homozygous. Genetic assays are, therefore, required to identify the genotype. Our AGameOfClones vector concept uses two clearly distinguishable transformation markers embedded in interweaved, but incompatible Lox site pairs. Cre-mediated recombination...

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Detalles Bibliográficos
Autores principales: Strobl, Frederic, Anderl, Anita, Stelzer, Ernst HK
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854464/
https://www.ncbi.nlm.nih.gov/pubmed/29543587
http://dx.doi.org/10.7554/eLife.31677

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854464/
https://www.ncbi.nlm.nih.gov/pubmed/29543587
http://dx.doi.org/10.7554/eLife.31677

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