Determination of Proteinaceous Selenocysteine in Selenized Yeast

A method for the quantitation of proteinaceous selenocysteine (SeCys) in Se-rich yeast was developed. The method is based on the reduction of the Se-Se and S-Se bridges with dithiotretiol, derivatization with iodoacetamide (carbamidomethylation), followed by HPLC-ICP MS. The chromatographic conditio...

Descripción completa

Detalles Bibliográficos
Autores principales: Bierla, Katarzyna, Lobinski, Ryszard, Szpunar, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855765/
https://www.ncbi.nlm.nih.gov/pubmed/29439473
http://dx.doi.org/10.3390/ijms19020543

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855765/
https://www.ncbi.nlm.nih.gov/pubmed/29439473
http://dx.doi.org/10.3390/ijms19020543

Ejemplares similares