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Evolved polymerases facilitate selection of fully 2′-OMe-modified aptamers

RNA or DNA aptamers with 2′-OMe-modifications have been pursued to increase resistance to nucleases, but have been difficult to identify because the OMe groups ablate polymerase recognition. We recently reported evolution of the thermostable DNA polymerases SFM4-6 and SFM4-9, which enable the effici...

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Detalles Bibliográficos
Autores principales: Liu, Zhixia, Chen, Tingjian, Romesberg, Floyd E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855981/
https://www.ncbi.nlm.nih.gov/pubmed/29568464
http://dx.doi.org/10.1039/c7sc03747c