Cargando…

Evolved polymerases facilitate selection of fully 2′-OMe-modified aptamers

RNA or DNA aptamers with 2′-OMe-modifications have been pursued to increase resistance to nucleases, but have been difficult to identify because the OMe groups ablate polymerase recognition. We recently reported evolution of the thermostable DNA polymerases SFM4-6 and SFM4-9, which enable the effici...

Descripción completa

Detalles Bibliográficos
Autores principales:	Liu, Zhixia, Chen, Tingjian, Romesberg, Floyd E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Royal Society of Chemistry 2017
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855981/ https://www.ncbi.nlm.nih.gov/pubmed/29568464 http://dx.doi.org/10.1039/c7sc03747c

Ejemplares similares

Evolution of Thermophilic DNA Polymerases for the Recognition and Amplification of C2’-Modified DNA
por: Chen, Tingjian, et al.
Publicado: (2016)

How the change of OMe substituent position affects the performance of spiro-OMeTAD in neutral and oxidized forms: theoretical approaches
por: Ashassi-Sorkhabi, Habib, et al.
Publicado: (2018)

Substituent effects on the pairing and polymerase recognition of simple unnatural base pairs
por: Hwang, Gil Tae, et al.
Publicado: (2006)

Using modified aptamers for site specific protein–aptamer conjugations
por: Wang, Ruowen, et al.
Publicado: (2016)

The Evolution of Classical Spiro-OMeTAD: Synthesis of Arylamine Endcapped Indenone Spirofluorene
por: Liu, Shihui, et al.
Publicado: (2022)

Solution-state structure of a fully alternately 2′-F/2′-OMe modified 42-nt dimeric siRNA construct
por: Podbevsek, Peter, et al.
Publicado: (2010)

UV ozone treatment for oxidization of spiro-OMeTAD hole transport layer
por: Hayashi, Ryotaro, et al.
Publicado: (2023)

Intracellular stability of 2′-OMe-4′-thioribonucleoside modified siRNA leads to long-term RNAi effect
por: Takahashi, Mayumi, et al.
Publicado: (2012)

From polymerase engineering to semi-synthetic life: artificial expansion of the central dogma
por: Sun, Leping, et al.
Publicado: (2022)

The selection of highly specific and selective aptamers using modified SELEX and their use in process analytical techniques for Lucentis bioproduction
por: Bhardwaj, Tanu, et al.
Publicado: (2020)

Carbon–carbon bond activation by B(OMe)(3)/B(2)pin(2)-mediated fragmentation borylation
por: Wang, Li, et al.
Publicado: (2021)

No place like Ome
por: Petsko, Gregory A
Publicado: (2002)

Reading and writing omes
por: Church, George M
Publicado: (2013)

A Novel Organic Dopant for Spiro-OMeTAD in High-Efficiency and Stable Perovskite Solar Cells
por: Guo, Ying
Publicado: (2022)

Selection and Biosensor Application of Aptamers for Small Molecules
por: Pfeiffer, Franziska, et al.
Publicado: (2016)

Rapid selection of aptamers based on protein microarray
por: Yu, Fang, et al.
Publicado: (2019)

[Ni(III)(OMe)]-mediated reductive activation of CO(2) affording a Ni(κ(1)-OCO) complex
por: Chiou, Tzung-Wen, et al.
Publicado: (2016)

Synthesis and hybridizing properties of P-stereodefined chimeric [PS]-{DNA:RNA} and [PS]-{DNA:(2′-OMe)-RNA} oligomers
por: Jastrzębska, Katarzyna, et al.
Publicado: (2022)

The effects of unnatural base pairs and mispairs on DNA duplex stability and solvation
por: Hwang, Gil Tae, et al.
Publicado: (2009)

'Oming in on RNA–protein interactions
por: Rinn, John L, et al.
Publicado: (2014)

Aptamer-Based Western Blot for Selective Protein Recognition
por: Wang, Yao, et al.
Publicado: (2020)

Analysis of Modified Nucleotide Aptamer Library Generated by Thermophilic DNA Polymerases
por: Percze, Krisztina, et al.
Publicado: (2020)

Selection of aptamers for AMACR detection from DNA libraries with different primers
por: Yang, Deng-Kai, et al.
Publicado: (2018)

Small-Molecule Binding Aptamers: Selection Strategies, Characterization, and Applications
por: Ruscito, Annamaria, et al.
Publicado: (2016)

RNA aptamers selected against DNA polymerase β inhibit the polymerase activities of DNA polymerases β and κ
por: Gening, Leonid V., et al.
Publicado: (2006)

Altered stoichiometry of an evolved RNA aptamer
por: Ohuchi, Shoji, et al.
Publicado: (2018)

The Biological Observation Matrix (BIOM) format or: how I learned to stop worrying and love the ome-ome
por: McDonald, Daniel, et al.
Publicado: (2012)

Strong electron acceptor additive based spiro-OMeTAD for high-performance and hysteresis-less planar perovskite solar cells
por: Wang, Shibo, et al.
Publicado: (2020)

A Semi-Synthetic Organism with an Expanded Genetic Alphabet
por: Malyshev, Denis A., et al.
Publicado: (2014)

In vitro optimization of 2′-OMe-4′-thioribonucleoside–modified anti-microRNA oligonucleotides and its targeting delivery to mouse liver using a liposomal nanoparticle
por: Takahashi, Mayumi, et al.
Publicado: (2013)

Boc-AzAla-Ala-OMe
por: Abbas, Cécile, et al.
Publicado: (2009)

Profiling the IgOme: Meeting the challenge()
por: Weiss-Ottolenghi, Yael, et al.
Publicado: (2014)

Selection of potential aptamers for specific growth stage detection of Yersinia enterocolitica
por: Shoaib, Muhammad, et al.
Publicado: (2020)

Aptamers 101: aptamer discovery and in vitro applications in biosensors and separations
por: Yang, Lucy F., et al.
Publicado: (2023)

Selection of DNA aptamers with two modified bases
por: Gawande, Bharat N., et al.
Publicado: (2017)

FeCl(3) as a low-cost and efficient p-type dopant of Spiro-OMeTAD for high performance perovskite solar cells
por: Gu, Xiaoyu, et al.
Publicado: (2018)

An organic hole-transporting material spiro-OMeTAD doped with a Mn complex for efficient perovskite solar cells with high conversion efficiency
por: Elawad, Mohammed, et al.
Publicado: (2021)

Amplifying fluorescence signal contrast of aptamer-modified microspheres inspired by whispering-gallery mode lasing
por: Kim, Sooyeon, et al.
Publicado: (2018)

LNA units present in [R(P)-PS]-(DNA#LNA) chimeras enhance the thermal stability of parallel duplexes and triplexes formed with (2′-OMe)-RNA strands
por: Jastrzębska, Katarzyna, et al.
Publicado: (2020)

Identification of a novel DNA aptamer that selectively targets lung cancer serum
por: Zhao, Yunwang, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_9vb16ud91rfh4oqkic7gr963uk